骨形成蛋白2/珍珠层粉/壳聚糖支架制备及生物性能研究

doi:10.13523/j.cb.20151101

中国生物工程杂志

2015, Vol. 35

Issue (11): 1-6 DOI: 10.13523/j.cb.20151101

研究报告

骨形成蛋白2/珍珠层粉/壳聚糖支架制备及生物性能研究

李婷¹, 孙静², 赵相哲¹, 连立强¹, 谢富强^1,2

1. 兰州大学口腔医学院兰州 730000;
2. 兰州大学第二医院兰州 730010

Preparation and Characterization Testing of Bone Morphogenetic Protein2/ Pearl Powder/Chitosan Porous Scaffolds

LI Ting¹, SUN Jing², ZHAO Xiang-zhe¹, LIAN Li-qiang¹, XIE Fu-qiang^1,2

1. School of Stomatology, Lanzhou University, Lanzhou 730000, China;
2. The Second Hospital of Lanzhou University, Lanzhou 730010, China

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摘要：

目的:制备骨形成蛋白2/珍珠层粉/壳聚糖复合多孔支架,观察支架生物性能。方法:采用冷冻干燥法制备骨形成蛋白2/珍珠层粉/壳聚糖多孔支架。用光学显微镜和扫描电子显微镜观察支架表面形貌及孔径大小,用比重瓶法检测支架孔隙率,热重分析探讨支架的热稳定性,用微力试验机进行压缩性能测试,并将支架与兔骨髓间充质干细胞共培养检测细胞黏附性能,将支架埋置大鼠皮下观察其炎症反应。结果与结论:制备的骨形成蛋白2/珍珠层粉/壳聚糖支架孔径大小为100~300μm,孔隙率为91.64%,压缩应力达3.37MPa,与细胞共培养贴附较好,有良好的组织相容性,提示该支架可做为组织工程支架材料应用于临床上骨组织缺损的修复。

关键词： 生物相容性; 壳聚糖; 珍珠层粉; 骨形成蛋白2

Abstract:

Objective: To prepare the bone morphogenetic protein- 2/pearl powder/chitosan composite scaffolds and observe biological properties. Methods: The bone morphogenetic protein-2/pearl powder/chitosan porous scaffolds were prepared by freeze-drying. The surface properties, the porosity, and the thermal stability of the stent were observed by light microscopy and scanning electron microscopy(SEM), pycnometer, and TGA respectively. Then the rabbit bone marrow mesenchymal stem cells were co-cultured with the stent to detect cell adhesion properties, and inflammatory reaction was observed by burying the stent in subcuticle of rats. Results and conclusion: The results showed that the pore size of bone morphogenetic protein 2/pearl powder/chitosan scaffold ranged between 100μm and 300μm with porosity being 91.64% and compressive stresses up to 3.37MPa. The stent,had suitable cell adhesion properties, and the histocompatibility is predominant, suggesting that the stent may be used as Bone Tissue Engineering materials applied for clinical repairment for bone tissue defects.

Key words: Nacre powder Biocompatibility Chitosan Bone morphogenetic protein 2

收稿日期: 2015-05-26 出版日期: 2015-11-24

ZTFLH:

R318

基金资助:

甘肃省自然科学基金资助项目(145RJZA149)

通讯作者: 孙静, 谢富强 E-mail: sunjing@lzu.edu.cn;xiefq@lzu.edu.cn

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引用本文:

李婷, 孙静, 赵相哲, 连立强, 谢富强. 骨形成蛋白2/珍珠层粉/壳聚糖支架制备及生物性能研究[J]. 中国生物工程杂志, 2015, 35(11): 1-6.

LI Ting, SUN Jing, ZHAO Xiang-zhe, LIAN Li-qiang, XIE Fu-qiang. Preparation and Characterization Testing of Bone Morphogenetic Protein2/ Pearl Powder/Chitosan Porous Scaffolds. China Biotechnology, 2015, 35(11): 1-6.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20151101 或 https://manu60.magtech.com.cn/biotech/CN/Y2015/V35/I11/1

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