聚己内酯/壳聚糖神经导管复合骨髓间充质干细胞促进大鼠坐骨神经损伤修复的研究

doi:10.13523/j.cb.20140206

中国生物工程杂志

2014, Vol. 34

Issue (2): 34-38 DOI: 10.13523/j.cb.20140206

研究报告

聚己内酯/壳聚糖神经导管复合骨髓间充质干细胞促进大鼠坐骨神经损伤修复的研究

刘奔, 张彩顺, 高绪斌, 李桦楠, 孔旭

辽宁医学院附属第一医院锦州 121001

Study on the Preparation of Polycaprolactone/chitosan Nerve Conduit Combined with Bone Marrow Mesenchymal Stem Cells to Promote Sciatic Nerve Injury Repair in Rats

LIU Ben, ZHANG Cai-shun, GAO Xu-bin, LI Hua-nan, KONG Xu

First Affiliated Hospital of Liaoning Medical College, Jinzhou 121001, China

全文: PDF(832 KB) HTML

摘要： 目的：观察聚己内酯/壳聚糖神经导管复合骨髓间充质干细胞修复大鼠坐骨神经缺损的效果。方法：将24只SD大鼠随机分为4组，制备右侧坐骨神经5mm缺损模型，A组聚己内酯/壳聚糖神经导管复合骨髓间充质干细胞移植组；B组聚己内酯神经导管复合骨髓间充质干细胞移植组；C组壳聚糖神经导管复合骨髓间充质干细胞移植组；D组自体神经移植组。术后每2周进行坐骨神经功能指数检测，12周时行电生理、腓肠肌湿重恢复率、组织学观察和免疫组织化学检测。结果：坐骨神经功能指数显示，A组运动功能恢复速度较B、C组快，但比D组慢。A组电生理和腓肠肌湿重恢复率的检测结果与C、D组相比无统计学意义（P>0.05），但优于B组（P<0.05）。组织学观察，A组再生神经纤维排列密集。S-100免疫组织化学结果表明A组有大量雪旺细胞增生。结论：聚己内酯/壳聚糖神经导管复合骨髓间充质干细胞能够促进周围神经损伤修复，效果与壳聚糖神经导管、自体神经相同，优于聚己内酯神经导管。

关键词： 周围神经再生; 神经导管; 聚己内酯; 壳聚糖; 骨髓间充质干细胞

Abstract: Objective:To observe the effects of polycaprolactone/chitosan(PCL/CS)nerve conduit combined with bone marrow mesenchymal stem cells(BMSCs)for repairing sciatic nerve defect in rats.Methods:Twenty four SD rats were made a model of 5mm right sciatic nerve defect in length and were randomly divided into four groups,group A:PCL/CS nerve conduit combined with BMSCs transplantation;group B:PCL nerve conduit combined with BMSCs transplantation;group C:CS nerve conduit combined with BMSCs transplantation;group D:autograft nerve.Sciatic function index(SFI)was evaluated every 2 weeks after surgery and tests of electrophysiology,the weight ratio of the gastrocnemius muscles,histological observation and immunohistochemistry were performed at 12 weeks postimplantation.Results:The recovery rate of motor function of group A was higher in comparison to group B and C but lower than that of group D as revealed by an SFI score.Compared the test results of electrophysiology and the weight ratio of the gastrocnemius muscles in group A with that in group C and D,there were not statistically significant(P>0.05),but that in group A better than that in group B (P<0.05).Histological observation:the regenerative nerve of group A lined up intensively.S-100 protein immunohistochemistry showed there were much more regenerating Schwann cells in group A.Conclusion:PCL/CS nerve conduit combined with BMSCs can promote peripheral nerve injury repair and its result is the same as CS nerve conduit and autograft nerve but better than PCL nerve conduit.

Key words: Peripheral nerve regeneration Nerve conduit Polycaprolactone Chitosan Bone marrow mesenchymal stem cells

收稿日期: 2013-08-15 出版日期: 2014-02-25

ZTFLH:

R745.42

基金资助: 辽宁省高等学校优秀人才支持计划（LJQ2013090）资助项目

通讯作者: 张彩顺 E-mail: zcs731009@163.com

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刘奔, 张彩顺, 高绪斌, 李桦楠, 孔旭. 聚己内酯/壳聚糖神经导管复合骨髓间充质干细胞促进大鼠坐骨神经损伤修复的研究[J]. 中国生物工程杂志, 2014, 34(2): 34-38.

LIU Ben, ZHANG Cai-shun, GAO Xu-bin, LI Hua-nan, KONG Xu. Study on the Preparation of Polycaprolactone/chitosan Nerve Conduit Combined with Bone Marrow Mesenchymal Stem Cells to Promote Sciatic Nerve Injury Repair in Rats. China Biotechnology, 2014, 34(2): 34-38.

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https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20140206 或 https://manu60.magtech.com.cn/biotech/CN/Y2014/V34/I2/34

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