大豆异黄酮生物合成关键酶及其代谢工程研究进展

中国生物工程杂志

2012, Vol. 32

Issue (07): 133-138

综述

大豆异黄酮生物合成关键酶及其代谢工程研究进展

陈宣钦, 张乐, 徐慧妮, 陈丽梅, 李昆志

昆明理工大学生物工程技术研究中心昆明 650500

Key Enzymes in Soybean Isoflavones Biosynthesis and Its Metabolic Engineering

CHEN Xuan-qin, ZHANG Le, XU Hui-ni, CHEN Li-mei, LI Kun-zhi

Biotechnology Research Center, Kunming University of Science and Technology, Kunming 650500, China

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摘要： 异黄酮是一类具有C-6/C-3/C-6骨架的二次代谢产物,具有抗氧化和抗肿瘤活性。异黄酮与黄酮类物质具有相似的苯丙烷生物合成途径。天然的绝大部分异黄酮分布在豆科植物中,目前在大豆中已经发现了超过12个异黄酮(苷)。大豆异黄酮的生物合成主要涉及三个关键的酶查尔酮合酶(CHS)、查尔酮异构酶(CHI)和异黄酮合酶(IFS)。总结了大豆异黄酮的提取分离方法和生物合成途径,着重综述了CHI、CHS、IFS生物学特征和功能及异黄酮的代谢工程研究。

关键词： 大豆异黄酮; CHI; CHS; IFS; 代谢工程

Abstract: Isoflavones were one class of secondary metabolites with C-6/C-3/C-6 skeleton, which possessed anti-oxidant and anti-tumor activities. Isoflavones and flavonoids had similar phenylpropanoid biosynthetic pathway. The majority of natural isoflavones occurred in leguminous plants. At present, more than 12 isoflavones were identified in soybean. The biosynthesis of soybean isoflvones mainly involved three key enzymes, which are CHS (chalcone synthase), CHI (chalcone isomerase), and IFS (isoflavone synthase). summarized The extraction and isolation methods and biosynthesis pathway of soybean isoflavones were summarized, and emphatically reviewed the biological characteristic and functions of CHI, CHS, and IFS as well as isoflovnes metabolic engineering were emphatically reviewed.

Key words: Soybean isoflavone CHI CHS IFS Metabolic engineering

收稿日期: 2011-12-02 出版日期: 2012-07-25

ZTFLH:

Q814

基金资助: 云南省自然科学基金(KKSA201126058);国家自然科学基金(31060258)资助项目

通讯作者: 李昆志 E-mail: likunzhikm@yahoo.com.cn

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引用本文:

陈宣钦, 张乐, 徐慧妮, 陈丽梅, 李昆志. 大豆异黄酮生物合成关键酶及其代谢工程研究进展[J]. 中国生物工程杂志, 2012, 32(07): 133-138.

CHEN Xuan-qin, ZHANG Le, XU Hui-ni, CHEN Li-mei, LI Kun-zhi. Key Enzymes in Soybean Isoflavones Biosynthesis and Its Metabolic Engineering. China Biotechnology, 2012, 32(07): 133-138.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2012/V32/I07/133

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