Advances in Genetically Engineered Animal Models of Chronic Obstructive Pulmonary Disease

doi:10.13523/j.cb.1909072

China Biotechnology

2020, Vol. 40

Issue (4): 59-68 DOI: 10.13523/j.cb.1909072

Advances in Genetically Engineered Animal Models of Chronic Obstructive Pulmonary Disease

LIU Di^1,²,ZHANG Hong-chun^2,^**(

)

1 Beijing University of Chinese Medicine, Beijing 100029, China
2 Department of TCM Pulmonary Diseases, China-Japan Friendship Hospital; Center of Respiratory Medicine, China-Japan Friendship Hospital; National Clinical Research Center for Respiratory Diseases, Beijing 100029, China

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Abstract

Chronic obstructive pulmonary disease (COPD) is a common chronic airway inflammatory disease with high morbidity and mortality in China, and it has caused heavy social and economic burden. It is reported that the incidence of COPD is closely related to genetic and environmental factors. Animal model is an important tool to study its pathogenesis, prevention, treatment and identify potential therapeutic targets and biomarkers. With the development of genetic engineering technology and the continuous discovery of related targets and genes of COPD, gene modified animal models are increasingly established and used in COPD research. In this paper, we searched published papers in PubMed to analyze the animal species and modeling methods of previous models of COPD. Then we data-mined the susceptibility genes of COPD and reviewed the susceptibility genes of different species by literature and database tool analysis. Finally, we integrated and listed the information and research progress of COPD genetic engineering mouse and rat models. Those informations are convenient for researchers and clinicians to reference and use. Thereafter, they can facilitate the research of pathogenesis and prevention methods of COPD.

Key words： Chronic obstructive pulmonary disease Animal model Genetic engineering

Received: 03 October 2019 Published: 18 May 2020

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Corresponding Authors: Hong-chun ZHANG E-mail: 13701226664@139.com

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Cite this article:

LIU Di,ZHANG Hong-chun. Advances in Genetically Engineered Animal Models of Chronic Obstructive Pulmonary Disease. China Biotechnology, 2020, 40(4): 59-68.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.1909072 OR https://manu60.magtech.com.cn/biotech/Y2020/V40/I4/59

Fig.1 Development trend of COPD animal model literature in PubMed

Fig.2 Species classification of COPD animal models (by number of literatures)

Fig.3 622 susceptibility genes associated with COPD (top 20)

靶基因 Target genes	靶基因全称 Target gene full name	遗传相关性 Genetic association	总体相关性 Overall association
FAM13A	家族序列相似性基因13A(family with sequence similarity 13 member A)	0.978 453	1
CHRNA3	胆碱能尼古丁受体α3(cholinergic receptor nicotinic alpha 3 subunit)	0.954 764	1
NPNT	肾连蛋白(nephronectin)	0.95	0.953 6
THSD4	I型血小板反应蛋白4(thrombospondin type 1 domain containing 4)	0.925 347	0.932 422
CHRNA5	胆碱能尼古丁受体α5(cholinergic receptor nicotinic alpha 5 subunit)	0.919 778	0.960 71
ADGRG6	粘附G蛋白偶联受体G6(adhesion G protein-coupled receptor G6)	0.906 82	0.912 22
HTR4	5-羟色胺受体4(5-hydroxytryptamine receptor 4)	0.876 396	0.886 893
ADAM19	ADAM金属肽酶19(ADAM metallopeptidase domain 19)	0.865 212	0.879 547
EEFSEC	硒特异延伸因子(eukaryotic elongation factor, selenocysteine-tRNA specific)	0.821 811	0.831 311
AGER	晚期糖基化终末产物特异性受体(advanced glycosylation end-product specific receptor)	0.807 588	0.823 249
CFDP1	颅面部发育蛋白1(craniofacial development protein 1)	0.799 618	0.804 618
TET2	甲基胞嘧啶双加氧酶2(tet methylcytosine dioxygenase 2)	0.787 572	0.791 272
RIN3	RAS相关/干扰蛋白3 (Ras and Rab interactor 3)	0.777 619	0.787 039
HHIP	Hedgehog相互作用蛋白(hedgehog interacting protein)	0.771 856	0.825 219
CHRNB4	胆碱能受体烟碱β4(cholinergic receptor nicotinic beta 4 subunit)	0.767 843	0.824 281
INTS12	整合因子复合体12(integrator complex subunit 12)	0.764 856	0.775 156
ARMC2	犰狳重复内含蛋白2(armadillo repeat containing 2)	0.727 875	0.727 875
ARHGAP42	Rho家族GTP酶活化蛋白42(Rho GTPase activating protein 42)	0.694 175	0.694 175
SERPINA1	丝氨酸蛋白酶抑制剂A1(serpin family A member 1)	0.666 944	0.698 373
CDC123	细胞分裂周期蛋白123(cell division cycle 123)	0.65	0.65

Table 1 Top 20 susceptibility genes associated with COPD in Open Targets Platform

Table 1 Result of retrieving susceptibility genes of COPD in RGD database

Fig.4 Wayne diagram of cross-over of susceptibility genes of COPD in human, mice and rats

Fig.5 Chronic obstructive pulmonary disease related genes and animal models in MGI (Cited:http://www.informatics.jax.org/humanDisease.shtml)

Table 3 Genetically engineered mice model of COPD in MGI

Table 5 Fam13a mutant mouse model

Table 6 Chronic obstructive pulmonary disease-related rat gene


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