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中国生物工程杂志

China Biotechnology
China Biotechnology
China Biotechnology  2018, Vol. 38 Issue (1): 78-87    DOI: 10.13523/j.cb.20180110
Orginal Article     
Construction of Male-sterility System Using Biotechnology and Application in Crop Breeding and Hybrid Seed Production
Suo-wei WU1,2,Xiang-yuan WAN1,2()
1 Advanced Biotechnology and Application Research Center, Institute of Biology and Agriculture, School of Chemistry and Biological Engineering,University of Science and Technology Beijing, Beijing 100024, China
2 Beijing Engineering Laboratory of Main Crop Bio-Tech Breeding, Beijing International Science and Technology Cooperation Base of Bio-Tech Breeding, Beijing Solidwill Sci-Tech Co. Ltd., Beijing 100192, China
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Abstract  

Male sterility plays an important role in hybrid vigor utilization and hybrid seed production in crops. The three-line system and two-line system based on cytoplasmic male sterility and photo-thermo sensitive male sterility had been widely used in crop hybrid seed production. But there are some limitations such as low efficiency of germplasm utilization, unstable fertility in variable environmental condition. In the last three decades, many artificial manipulations of male sterility in plants have been accomplished by using genetic engineering or biotechnology strategies. The reported approaches used for generating artificial biotechnology male-sterile lines in the main three crops such as maize, rice and wheat are outlined. Especially, detail the multi-control sterility (MCS) system in maize designed by our laboratory recently is described. This will give some insights on the commercial application of male sterility in crop breeding and hybrid seed production.

Key words： Male sterility      Genetic engineering      Hybrid vigor      Maize      Rice      Wheat     
Received: 30 November 2017      Published: 31 January 2018
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Suo-wei WU
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Suo-wei WU,Xiang-yuan WAN. Construction of Male-sterility System Using Biotechnology and Application in Crop Breeding and Hybrid Seed Production. China Biotechnology, 2018, 38(1): 78-87.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20180110     OR     https://manu60.magtech.com.cn/biotech/Y2018/V38/I1/78

Fig.1 The technological strategy of maize multi-control sterility system
No.Gene nameCoded proteinsReferences
1ms1, male sterility1LOB/LBD protein 30[10]
2ms7, male sterility7PHD-finger protein[2, 11]
3ms30, male sterility30GDSL-Lipase[12]
4ms33, male sterility33GPAT protein[13]
Table 1 The cloned male sterility genes for construction of the MCS system in maize in our lab
ConstructsPromoter-gene combinationReferences
pMCS0101p35S∷Bar//pZmMs1:ZmMs1//pZm13∷Dam//pPG47∷Bt1:ZmAA//pLTP2∷mCherry[14]
pMCS0102p35S∷Bar//pPG47∷Bt1:ZmAA//pZmMs1∷ZmMs1//pLTP2∷DsRed2[14]
pMCS0701p35S∷Bar//pZmMs7:ZmMs7//pZm13∷Dam//pPG47∷Bt1:ZmAA//pLTP2∷mCherry[15]
pMCS0702p35S∷Bar//pPG47∷Bt1:ZmAA//pZmMs7∷ZmMs7//pLTP2∷DsRed2[15]
pMCS0703p35S∷Bar//pPG47∷Bt1:ZmAA//pZm13∷Dam//pZmMs7∷ZmMs7//pLTP2∷DsRed2[15]
pMCS3001p35S∷Bar//pZmMs30:ZmMs30//pZm13∷Dam//pPG47∷Bt1:ZmAA//pLTP2∷mCherry[16]
pMCS3002p35S∷Bar//pPG47∷Bt1:ZmAA//pZmMs30∷ZmMs30//pLTP2∷DsRed2[16]
pMCS3003p35S∷Bar//pPG47∷Bt1:ZmAA//pZm13∷Dam//pZmMs30∷ZmMs30//pLTP2∷DsRed2[16]
pMCS3301p35S∷Bar//pZmMs33:ZmMs33//pZm13∷Dam//pLTP2∷mCherry
pMCS3302p35S∷Bar//pPG47∷Bt1:ZmAA//pZm13∷Dam//pZmMs33∷ZmMs33//pLTP2∷DsRed2
Table 2 Commercial constructs for development of the MCS system using male sterility genes
Fig.2 Phenotype of three ZmMs7 transgenic maize male-sterile maintainer lines (BC2F1)(a), (c) and (e) Ear photos of the three male-sterile maintainer lines (1601#, 1602# and 1603#) under bright light (BL) (b), (d) and (f) The ear photos corresponding to (a), (c) and (e) Under a red fluorescence (RF) filter, respectively. These lines are the BC2F1 progeny of the elite line M0701-2 crossed with ms7-6007 mutant and then backcrossed to inbred line Zheng58. BC2F1 is the second backcross generation. Bars = 1cm
Fig.3 The panicle phenotype of three rice male-sterility maintainer lines(a), (b) and (c) the panicles of three independent rice male-sterility maintainer lines in the paddy field. In each panicle. the red fluorescence seeds are transgenic male-sterility maintainer lines, the normal color seeds are non-transgenic male-sterility lines
Fig.4 The experimental design of the split-gene system for hybrid wheat seed production
Fig.5 The schematic representation of the MCS and SPT process with commercial hybrid seed production
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