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中国生物工程杂志

China Biotechnology
China Biotechnology
China Biotechnology  2014, Vol. 34 Issue (9): 94-101    DOI: 10.13523/j.cb.20140914
    
Identification of an Alginate Lyase Producing Strain Halomonas sp. WF6 and Fermentation Optimization
LI Heng, ZHU Si-ting, LIU Xu-mei, GONG Jin-song, JIANG Min, XU Zheng-hong, SHI Jin-song
School of Pharmaceutical Science, Jiangnan University, Wuxi 214122, China
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Abstract  

An efficient alginate-degrading strain isolated from rotten seaweed was identified. With the results of morphological properties, physiological characteristics as well as 16S rDNA sequencing, the strain was identified as Halomonas sp. WF6. The fermentation medium component was optimized by both single factor and orthogonal test, which was composed of sodium alginate 6.0 g/L, peptone 5.0 g/L, yeast extract 2.5 g/L, NaCl 30 g/L, K+ 5 mmol/L. This strain was cultivated at 25℃ for 39 h with optimized conditions of initial pH 8.0, inoculum size 2% and liquid volume 30 ml/250 ml, the alginate lyase activity was increased to 117.66 U/ml, which was 2.1-fold compared with the control. The enzymatic hydrolysates of sodium alginate mainly included di- and tri-alginate oligosaccharides.

Key words： Alginate lyase      Halomonas sp.      Idendification      Optimization     
Received: 03 June 2014      Published: 25 September 2014
ZTFLH:  Q93  
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Cite this article:

LI Heng, ZHU Si-ting, LIU Xu-mei, GONG Jin-song, JIANG Min, XU Zheng-hong, SHI Jin-song. Identification of an Alginate Lyase Producing Strain Halomonas sp. WF6 and Fermentation Optimization. China Biotechnology, 2014, 34(9): 94-101.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20140914     OR     https://manu60.magtech.com.cn/biotech/Y2014/V34/I9/94


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