The Elementary Identification of Antagonistic Bacteria against Botrytis cinerea and Improving Biocontrol Effect Through Genetic Modification with Chitinase Gene

China Biotechnology

2010, Vol. 30

Issue (02): 71-76 DOI:

The Elementary Identification of Antagonistic Bacteria against Botrytis cinerea and Improving Biocontrol Effect Through Genetic Modification with Chitinase Gene

1.Institute of Life Science Northwest University, Xi’an 710069，China
2.College of Bioengeering Henan University of Technology，Zhengzhou 450001，China

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Abstract

Fh antagonist to Botrytis cinerea was preserved in laboratory. Through a series of morphological and biochemical characteristics, Fh belongs to Bacillus. In order to enhance the inhibitory effect of this strain, a 6.4 kb DNA fragment containing chitinase gene from pUC1965 was inserted into vector pBE2 to construct recombination plasmids called pBE2-chib . A new strain named Fh-chib was formed by transferring the recombination plasmids into Bacillus Fh. The PCR identification of chitinase gene and chitin plate culture confirmed that Chitinase gene was transferred successfully into the wild strain Fh. The original chitinase activity of Fh-chib using the vector pBE2 was 4.06U/ml. Fh-chib had an increased inhibitory effect against Botrytis cinerea on tomato by 23.2% through chitin plate culture.

Key words： Bacillus Chitinase Botrytis cinerea Transfer Biocontrol

Received: 10 July 2009 Published: 26 February 2010

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	HAN Wen-Xia
	CHEN Li
	CHEN Liang
	GONG Ji
	CHEN Wu-Ling

Cite this article:

HAN Wen-Xia, CHEN Li, CHEN Liang, GONG Ji, CHEN Wu-Ling. The Elementary Identification of Antagonistic Bacteria against Botrytis cinerea and Improving Biocontrol Effect Through Genetic Modification with Chitinase Gene. China Biotechnology, 2010, 30(02): 71-76.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2010/V30/I02/71

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