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| Expression of hsRAGE-IgG Fc Fusion Protein and Its Bioactivity |
| ZHANG Jing1,MO Gui-ling1,ZHANG Qing-you1,SU Zhi-jian2,XU Hua1,HUANG Ya-dong2,ZHENG Qing1 |
1.Pharmacy College,Jinan University,Guanzhou 510632,China
2.Institutes of Life and Health Engineering,Jinan University,Guanzhou 510632,China |
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Abstract The human soluble receptor for advanced glycation end products (hsRAGE) fused with the immunoglobulinin G Fc fragment (IgG Fc) was expressed in Rosetta(DE3) by IPTG induction.The result of SDS-PAGE analysis indicated the hsRAGE-IgG Fc fusion protein was expressed in the form of inclusion body.The expression level of the fusion protein was about 40% of the total cellular protein.After renaturation and purification,a purity of about 96% of the fusion protein was obtained.Result of Western blotting indicates the fusion protein can specifically combine with sRAGE antibody and significantly inhibit the over expression of NF-κB p65 of ECV-304 cells induced by AGEs.
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Received: 08 January 2010
Published: 12 June 2010
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