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| Screening, Identification and Virulence Factor Determination of the Bacteria with Nematicidal Activity to Bursaphelenchus xylophilus |
| NIU Qiu-hong,DONG Bing-xue,HUANG Si-liang,HUI Feng-li,KE Tao,ZHANG Lin |
| School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, China |
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Abstract One hundred and ninety eight bacteria were isolated and tested for their nematicidal activity from the soil in Nanyang, Henan Province, China. Six strains which had nematicidal activity to Bursaphelenchus xylophilus were found. A bacterium, named Bacillus sp. strain NS-3, showed the highest nematicidal activity to B xylophilus. The bacterium was classified by phenotypic and genotypic characteristics, which belonged to Bacillus genus. The culture supernatant and the crude extracellular protein extract from the strain NS-3 separately killed 50 % and 100 % of the tested nematodes (B. xylophilus) within 48 h, which suggested their high toxic activity toward the nematodes. Most of the dead nematodes were degraded and only minor fragments were left. However, the crude protein extract lost nematicidal activity after boiled, which showed the namaticidal materials produced in the cultural filtrate by the bacterium was not stable to the heat.
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Received: 15 January 2010
Published: 25 August 2010
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| ［1］ 董锦艳, 李铷, 张克勤. 松材线虫生物防治研究进展. 植物保护, 2005, 31 (5) : 915. Dong J Y, Li R, Zhang K Q. Plant Protection, 2005, 31 (5): 915.
［2］ Yang J K, Li J, Liang L M, et al. Cloning and characterization of an extracellular serine protease from the nematodetrapping fungus Arthrobotrys conoides. Arch Microbiol, 2007, 188: 167174.
［3］ 茆振川.防治根结线虫的原核生防菌的筛选及作用.杨凌: 西北农林科技大学, 2004. Mao Z C. Screening and function of biocontrol microbes against root knot nematodes . Yangling: Northwest SciTech University of Agriculture and Forestry, 2004.
［4］ 牛秋红, 黄晓玮, 徐进, 等. 细菌在线虫生防中应用的研究进展.生物技术, 2006 , 16 (1) : 9095. Niu Q H, Huang X W, Xu J, et al. Biotechnology, 2006, 16 (1): 9095.
［5］ Niu Q H, Huang X W, Zhang L, et al. Functional identification of the gene bace16 from nematophagous bacterium Bacillus nematocida. Appl Microbiol Biotechnol, 2007, 75(1): 141148.
［6］ Tian B Y, Yang J K, Zhang K Q. Bacteria used in biocontrol nematode: potential, development and perspective. FEMS Microbiol Ecol, 2007, 61: 197213.
［7］ Siddiqui I A, Haas D, Heeb S. Extracellular protease of Pseudomonas fluorescens CHA0, a biocontrol factor with activity against the rootknot nematode Meloidogyne incognita. Appl Environ Microbiol, 2005, 71(9): 56465649.
［8］ Wei J Z, Hale K, Carta L, et al. Bacillus thuringiensis crystal proteins that target nematodes. Proc Natl Acad Sci USA, 2003, 100(5): 27602765.
［9］ 海外传真. 农药, 2007, 46 (9) : 647. Overseas fax. Pesticide, 2007, 46 (9): 647.
［10］ Gray N F. Ecology of nematophagous fungi: comparison of the soil sprinkling method with the Baerman funnel technique in the isolation of endoparasites. Soil Biol Biochem, 1984, 16: 81~83.
［11］ 黄秀梨. 微生物学实验指导. 北京: 高等教育出版社, 2004. Huang X L. Experimental Guidance of Microbiology. Beijing: Higher Education Press, 2004.
［12］ Xu P, Li W J, Xu L H, et al. A microwavebased method for genomic DNA extraction from Actinomycetes, Microbiology, 2003, 30 (4): 7375.
［13］ Higgins D G, Bleasby A J, Fuchs R CLUSTAL V: Improved software for multiple sequence alignment. Comput Appl Biosci, 1992, 8: 189191.
［14］ Thompson J D, Gibson T J, Plewniak F, et al. The Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 1997, 24: 48764882.
［15］ Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequence. J Mol Evol, 1980, 16:111120.
［16］ Saitou N, Nei M. The neighborjoining method: a new method for reconstructing phylogenetic tree. Mol Biol Evol, 1987, 4: 406425.
［17］ Kumars S, Tamura K, Nei M. MEGA 3: Integrated software for molecular evolutionary genetics analysis and sequence alignment. Briefings in Bio informatics, 2004, 5: 150163.
［18］ Stirling G R, Wachtel M F. Mass production of Bacillus penetrans for the biological control of rootknot nematodes. Nematologica, 1980, 26: 308312.
［19］ KerenZur M, Antonov J, Bercovitz A, et al. Bacillus firmus formulations for the safe control of rootknot nematodes. In: Proceedings of the Brighton Crop Protection Conference on Pests and Diseases, 2000, vol. 2A, 4752.
［20］ Giannakou I O, Karpouzas D G. Demetra ProphetouAthanasiadou. A novel nonchemical nematicide for the control of rootknot nematodes. Applied Soil Ecology, 2004, 26: 6979.
［21］ Soad A A, Xie G L, Li B, et al. Comparative performance of Bacillus spp. in growth promotion and suppression of tomato bacterial wilts caused by Ralstonia solanacearum. Journal of Zhejiang University (Agric & Life Sci), 2004, 30(6): 603610.
［22］ Huang X W, Tian B Y, Niu Q H, et al. An extracellular protease from Brevibacillus laterosporus G4 without parasporal crystals can serve as a pathogenic factor in infection of nematodes. Res Microbiol, 2005, 156: 719727.
［23］ Lian L H, Tian B Y, Xiong R, et al. Proteases from Bacillus: a new insight on the mechanism of action for rhizobacterial suppression of nematode populations. Letters in Applied Mircobiology, 2007, 45: 262269.
［24］ Chen X H, Koumoutsi A, Scholz R, et al. Comparative analysis of the complete genome sequence of the plant growthpromoting bacterium Bacillus amyloliquefaciens FZB42. Nat Biotechnol, 2007, 25: 10071014. |
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