中国生物工程杂志

As lignin is a major structural component of plant cells, modification in lignin content may have ecological implications. Taking different transgenic cottons as test materials by a pot experiment, the lignin contents, POD and PAL activities in different cotton tissues were studied in this thesis and the changes of lignin metabolism in transgenic cottons were indicated. The results showed that the lignin contents in different plant tissues of transgenic Bt cottons were higher than that in their controls at the degree from 2.09% to 9.45%, and the differences among treatments were not significant. But the lignin contents in different plant tissues of transgenic Bt+CpTI cottons were lower than that in their controls at the degree from 9.19% to 31.95%, and only the difference in roots between transgenic and non-transgenic treatments was significant. In comparing with conventional cottons, POD and PAL activities in transgenic cottons had some changes in certain varieties and tissues. To transgenic crops, unintended effects would be more uncertain and complex along with diversification of transgenic genes.