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中国生物工程杂志

China Biotechnology
China Biotechnology  2015, Vol. 35 Issue (9): 28-34    DOI: 10.13523/j.cb.20150905
    
Comparsion of the Effects of pta and ack Deletion on Physiological Metabolism of L-arginine-producing Strain Corynebacterium crenatum
WAN Fang1, ZHANG Bin1, CHEN Min-liang2, CHEN Jin-cong2, CHEN Xue-lan1
1 College of Life Science, Jiangxi Normal University, Nanchang 330022, China;
2 College of Life Science, Nanchang University, Nanchang 330031, China
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Abstract  

The disruption of competitive metabolic pathways is conducive to the carbon flux converted into the purpose amino acid biosynthetic pathway. In the present study, based on the prediction of genome-scale metabolic network model, markless knock-out technology to construct separately recombinant strains C. crenatum MT-M4 △pta and C. crenatum MT-M4 △ack which led to interdict the acetic acid biosynthetic pathway were employed. As shake-flask fermentation results shown, L-arginine production of C. crenatum MT-M4 △pta was significantly increased by 25.60% higher than that of original strain, reached to 15.46g/L with glucose transformation rate increased by 29.41%. L-arginine production of C. crenatum MT-M4 △ack was 13.82g/L which was 12.81% higher than that of original strain, with glucose transformation rate increased by 26.02%. In addition, the growth of C. crenatum MT-M4 △pta and C. crenatum MT-M4 △ack was increased by 9.19% and 7.71%, respectively. Thus, pta and ack deletion was not only beneficial to the improvement of L-arginine production, but also conducive to the cell growth. However, pta deletion compared with ack deletion was more conducive to L-arginine accumulation.



Key wordsC. crenatum      L-arginine      Markerless knock-out technology      Genome-scale metabolic network model      Physiological metabolism     
Received: 18 May 2015      Published: 25 September 2015
ZTFLH:  Q812  
Cite this article:

WAN Fang, ZHANG Bin, CHEN Min-liang, CHEN Jin-cong, CHEN Xue-lan. Comparsion of the Effects of pta and ack Deletion on Physiological Metabolism of L-arginine-producing Strain Corynebacterium crenatum. China Biotechnology, 2015, 35(9): 28-34.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20150905     OR     https://manu60.magtech.com.cn/biotech/Y2015/V35/I9/28


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