Gene Coloning, Expression and Activity Assay of Survivin

China Biotechnology

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Gene Coloning, Expression and Activity Assay of Survivin

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Abstract Objective To study the expression and the function of survivin. Results The expression of survivin in embryo spleen, embryo kidney, embryo liver and in many cancer tissue was determined by RT-PCR. Construct the engineered Escherischia coli expressing human survivin and identify the expressed human survivin by Westen-blot. The combination activity of Survivin and RhSmac was determined in vitro. L929 cells transferred with Survivin can survive longer than which transferred with BSA.

Key words： expression Activity assay drug Screening surviving

Received: 27 October 2005 Published: 25 May 2006

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. Gene Coloning, Expression and Activity Assay of Survivin. China Biotechnology, 2006, 26(05): 54-57.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2006/V26/I05/54

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