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| Development and Application of L-Asparaginase with Better Performance and Advances in Recombinant Expression |
| ZENG Jie |
| Jiangsu Hengrui Medicine Co., Ltd., National Engineering Technology Research Center of Targeted Drugs, China, Lianyungang 222047, China |
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Abstract L-asparaginase is an anti-neoplastic agent, used in acute lymphoblastic leukemia(ALL) and lymphosarcoma chemotherapy. However, the application in clinic therapeutics is obstructed by its high costs of production. Nevertheless, the expression system of recombinant proteins have solved this problem perfectly. The intrinsic low-rate glutaminase activity of L-asparaginase, however, causes serious side-effects. In addition, L-asparaginase from bacterial origin can cause hypersensitivity in the long-term used, leading to allergic reactions and anaphylaxis. The search for new L-asparaginase sources, which shows less adverse effects, has become the focus of scholar studies. A review of recent progress in development of L-asparaginase with better performance are presented. Furthermore, the successful expression of recombinant L-asparaginase were aslo summarized and analyzed, and the potential perspective of L-asparaginase to be used in clinic therapeutics and food industry was discussed.
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Received: 02 May 2017
Published: 15 November 2017
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