Preparation and Wound Repair of Keratin/Sodium Alginate/Polyacrylamide Hydrogel Skin Dressing

doi:10.13523/j.cb.2103033

China Biotechnology

2021, Vol. 41

Issue (8): 17-24 DOI: 10.13523/j.cb.2103033

Preparation and Wound Repair of Keratin/Sodium Alginate/Polyacrylamide Hydrogel Skin Dressing

YUAN Xiao-jing¹,YIN Hai-meng¹,FAN Xiao-wei¹,HE Jun-lin¹,HAO Shi-lei²,JI Jin-gou^1,^*(

)

1 College of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331,China
2 College of Bioengineering, Chongqing University, Chongqing 401331,China

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Abstract

In order to obtain a more ideal skin wound repair dressing, human hair keratin (KTN) is compounded on the basis of sodium alginate (SA) and polyacrylamide (PAM) hydrogel to prepare KTN/SA/PAM hydrogel glue skin dressing.It was characterized by an electronic universal testing machine, scanning electron microscope, etc. The results showed that the tensile strength of the KTN/SA/PAM hydrogel skin dressing was 42.41 kPa and the elastic modulus was 11.19 kPa, which were close to human skin tissue; The adhesion of the skin can be as high as 5.1 kPa, and the water absorption rate in 2 hours is 144.3%, which satisfy the basic requirements of skin wound repair dressing. Further rat skin wound repair experiments show that KTN/SA/PAM hydrogel skin dressings have better repairing ability than commercially available wound tapes and SA/PAM hydrogels. Histological analysis of wound skin tissue sections shows that after KTN/SA/PAM skin dressing treatment, the immature tissue areas of the wound were the smallest and the collagen fibers were arranged most neatly. After 14 days, the degree of wound healing was almost similar to that of normal skin. The results indicated that KTN/SA/PAM skin dressing may be a more promising skin wound repair dressing.

Key words： Keratin Sodium alginate/polyacrylamide hydrogel Skin dressing Wound healing

Received: 13 March 2021 Published: 31 August 2021

ZTFLH:

Q946.1

Corresponding Authors: Jin-gou JI E-mail: 15883282@qq.com

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	Xiao-jing YUAN
	Hai-meng YIN
	Xiao-wei FAN
	Jun-lin HE
	Shi-lei HAO
	Jin-gou JI

Cite this article:

YUAN Xiao-jing,YIN Hai-meng,FAN Xiao-wei,HE Jun-lin,HAO Shi-lei,JI Jin-gou. Preparation and Wound Repair of Keratin/Sodium Alginate/Polyacrylamide Hydrogel Skin Dressing. China Biotechnology, 2021, 41(8): 17-24.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.2103033 OR https://manu60.magtech.com.cn/biotech/Y2021/V41/I8/17

Fig.1 Adhesion strength between skin dressings and porcine skin with bloodby different KTN concentrations **** P≤0.000 1, * P≤0.05

Fig.2 Adhesion strength between skin dressings and porcine skin with blood modified by different volumes of KTN solution **** P≤0.0001, *** P≤0.001 and * P≤0.05

Fig.3 SEM image of different materials (a)KTN/SA/PAM skin dressings (b)Partial enlarged KTN/SA/PAM skin dressings (c)SA/PAM hydrogels

Fig.4 Stress-strain curves of SA/PAM and KTN/SA/PAM skin dressings

Fig.5 Water absorption performance of KTN/SA/PAM skin dressings and SA/PAM hydrogel

Fig.6 Defective skin healing (a)Wounds on the day of surgery and 1, 5, 7, 14 days after surgery (b)Changes in wound closure area after surgery *** P≤0.001, ** P≤0.01 and * P≤0.05

Fig.7 H&E staining statistics chart (a)H&E tissue staining image of skin tissue at each time point (original magnification 100×), the white dashed line indicates the boundary of the epidermal layer, the black asterisk indicates the formation of blood vessels, and the red arrow indicates the presence of skin appendages (b)The thickness of the epidermal layer of the healed wound (c)The area of the skin adnexa of the healed wound (d)The area of the blood vessel of the healed wound *** P≤0.0001, ** P≤0.01 and * P≤0.05


[1]	Elieh-Ali-komi D, Hamblin M R. Chitin and chitosan: production and application of versatile biomedical nanomaterials. International Journal of Advanced Research, 2016, 4(3):411-427. pmid: 27819009

[2]	Zhang Y J, Gao B, Liu X W. Topical and effective hemostatic medicines in the battlefield. International Journal of Clinical and Experimental Medicine, 2015, 8(1):10-19.

[3]	Jabeen S, Islam A, Ghaffar A, et al. Development of a novel pH sensitive silane crosslinked injectable hydrogel for controlled release of neomycin sulfate. International Journal of Biological Macromolecules, 2017, 97:218-227. doi: 10.1016/j.ijbiomac.2017.01.014

[4]	Ullah F, Othman M B H, Javed F, et al. Classification, processing and application of hydrogels: A review. Materials Science & Engineering C, Materials for Biological Applications, 2015, 57:414-433. doi: 10.1016/j.msec.2015.07.053

[5]	Behrns K. Tissue adhesives in clinical medicine. Journal of Gastrointestinal Surgery, 2006, 10(8):1185. doi: 10.1016/j.gassur.2006.04.014

[6]	Esparza Y, Ullah A, Boluk Y, et al. Preparation and characterization of thermally crosslinked poly(vinyl alcohol)/feather keratin nanofiber scaffolds. Materials & Design, 2017, 133:1-9.

[7]	Esparza Y, Bandara N, Ullah A, et al. Hydrogels from feather keratin show higher viscoelastic properties and cell proliferation than those from hair and wool keratins. Materials Science and Engineering: C, 2018, 90:446-453. doi: 10.1016/j.msec.2018.04.067

[8]	Wang J, Hao S L, Luo T T, et al. Feather keratin hydrogel for wound repair: Preparation, healing effect and biocompatibility evaluation. Colloids and Surfaces B: Biointerfaces, 2017, 149:341-350. doi: S0927-7765(16)30759-7 pmid: 27792983

[9]	Sun J Y, Zhao X H, Illeperuma W R K, et al. Highly stretchable and tough hydrogels. Nature, 2012, 489(7414):133-136. doi: 10.1038/nature11409

[10]	Kuang J, Yuk K Y, Huh K M. Polysaccharide-based superporous hydrogels with fast swelling and superabsorbent properties. Carbohydrate Polymers, 2011, 83(1):284-290. doi: 10.1016/j.carbpol.2010.07.052

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