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| Subcellular Localization and Functional Studying of SCBP60g in Arabidopsis |
| WAN Yong-qing1, LI Rui-li1,2, ZOU Bo1, WAN Dong-li1,3, WANG Rui-gang1, LI Guo-jing 1 |
1. College of Life Sciences, Inner Mongolia Agricultural University, Hohhot 010018 China;
2. Ulanqab Centers for Diseases Controland Prevention, Ulanqab 012000, China;
3. Grassland Research Institute, Chinese Academy of Agricultural Sciences, Hohhot 010010, China |
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Abstract The GFP fused SCBP60g constructs and SCBP60g complementation constructs were made by Gateway cloning technology, and the transgenic plants were obtained. Subcellular localization SCBP60g was observed by confocal laser-scanning microscope, and the function of plant defense against pathogens was examined by in planta bacterial growth assay. In addition, SCBP60g overexpression vector was constructed and the sensitivity to ABA of the transgenic plants was also examined. The results showed:SCBP60g protein localized in the nuclei; SCBP60g failed to restore the sensitivity of cbp60g-1 mutant to Pseudomonas syringae; overexpression of SCBP60g did not affect the response of Arabidopsis to ABA. These results indicated that SCBP60g was not involved in Arabidopsis thaliana response to pathogens and ABA.
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Received: 25 June 2013
Published: 25 September 2013
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