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中国生物工程杂志

China Biotechnology
China Biotechnology  2014, Vol. 34 Issue (5): 30-38    DOI: 10.13523/j.cb.20140505
    
Transcriptome Analysis of Arabidopsis thaliana and Changes of Glucosinolates Metabolism Pathway Induced by Flg22
YU Xin-xin, GAO Jin-jun, LI Yong, LI Jing
Northeast Agricultural University, Key Laboratory of Agricultural Biological Functional Genes, Harbin 150030, China
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Abstract  

Flg22 is a highly conserved amino acid sequence in the N terminal of bacterial flagellum protein, which can induce plant natural immune response. In order to fully understand systemic responses of plant to bacterial pathogens, two sequencing libraries of Arabidopsis seedlings treated and not treated with flg22 were constructed and sequenced using Illumina Hiseq2000. Comparison of the two samples showed 1 200 differentially expressed genes (DEGs), including 290 down-regulated and 910 up-regulated genes. GO functional enrichment and KEGG pathway enrichment analysis revealed that the DEGs were associated with energy metabolism, amino acid metabolism and biosynthesis of secondary metabolites. Glucosinolates are important secondary metabolites involved in innate immune response against pathogen, therefore it is of necessary to analyze glucosinolates metabolism pathway. After flg22 treatment, genes involved in indolic glucosinolate biosynthesis pathway were up-regulated significantly,which is further demonstrated by Real Time RT-PCR, while aliphatic glucosinolate pathway almost had no change, indicating the important role of indolic glucosinolates in plant defense responses. This research provided numerous genetic data to deeply understand pathogen induced defense and the contribution of indolic glucosinolates.



Key wordsArabidopsis thaliana      flg22      High-throughput sequencing      Innate immune      Glucosinolates     
Received: 13 January 2014      Published: 25 May 2014
ZTFLH:  Q945  
Cite this article:

YU Xin-xin, GAO Jin-jun, LI Yong, LI Jing. Transcriptome Analysis of Arabidopsis thaliana and Changes of Glucosinolates Metabolism Pathway Induced by Flg22. China Biotechnology, 2014, 34(5): 30-38.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20140505     OR     https://manu60.magtech.com.cn/biotech/Y2014/V34/I5/30


[1] Jones J D G, Dangl J L. The plant immune system. Nature, 2006,444(7117) : 323-329.

[2] Aziz A, Heyraud A, Lambert B. Oligogalacturonide signal transduction, induction of defense-related responses and protection of grapevine against Botrytis cinerea. Planta, 2004, 218(5): 767-774.

[3] Ferrari S, Galletti R, Denoux C, et al. Resistance to Botrytis cinerea induced in Arabidopsis by elicitors is independent of salicylic acid, ethylene, or jasmonate signaling but requires Phytoalexin Deficient3. Plant physiology, 2007, 144(1): 367-379.

[4] Zipfel C, Robatzek S, Navarro L, et al. Bacterial disease resistance in Arabidopsis through flagellin perception. Nature, 2004, 428(6984): 764-767.

[5] Felix G, Duran J D, Volko S, et al. Plants have a sensitive perception system for the most conserved domain of bacterial flagellin. The Plant Journal, 1999, 18(3): 265-276.

[6] Gómez-Gómez L, Boller T. FLS2: An LRR receptor-like kinase involved in the perception of the bacterial elicitor flagellin in Arabidopsis. Molecular cell, 2000, 5(6): 1003-1011.

[7] Chinchilla D, Bauer Z, Regenass M, et al. The Arabidopsis receptor kinase FLS2 binds flg22 and determines the specificity of flagellin perception. The Plant Cell Online, 2006, 18(2): 465-476.

[8] Chinchilla D, Zipfel C, Robatzek S, et al. A flagellin-induced complex of the receptor FLS2 and BAK1 initiates plant defence. Nature, 2007, 448(7152): 497-500.

[9] Bari R, Jones J D G. Role of plant hormones in plant defence responses. Plant molecular biology, 2009, 69(4): 473-488.

[10] Asai T, Tena G, Plotnikova J, et al. MAP kinase signalling cascade in Arabidopsis innate immunity. Nature, 2002, 415(6875): 977-983.

[11] Mikkelsen M D, Petersen B L, Glawischnig E, et al. Modulation of CYP79 genes and glucosinolate profiles in Arabidopsis by defense signaling pathways. Plant Physiology, 2003, 131(1): 298-308.

[12] Yan X, Chen S. Regulation of plant glucosinolate metabolism. Planta, 2007, 226(6): 1343-1352.

[13] Clay N K, Adio A M, Denoux C, et al. Glucosinolate metabolites required for an Arabidopsis innate immune response. Science, 2009, 323(5910): 95-101.

[14] Agerbirk N, De Vos M, Kim J H, et al. Indole glucosinolate breakdown and its biological effects. Phytochemistry Reviews, 2009, 8(1): 101-120.

[15] Mikkelsen M D, Petersen B L, Olsen C E, et al. Biosynthesis and metabolic engineering of glucosinolates. Amino Acids, 2002, 22(3): 279-295.

[16] Langmead B, Trapnell C, Po PM, et al. Ultrafast and memory-efficient alignment of short DNA sequences to the human genome. Genome Biol, 2009, 10(3): R25

[17] Trapnell C, Pachter L, Salzberg S L. TopHat: discovering splice junctions with RNA-Seq. Bioinformatics, 2009, 25(9): 1105-1111.

[18] Anders S, Huber W. Differential expression of RNA-Seq data at the gene level-the DESeq package. 2012.

[19] Mikkelsen M D, Petersen B L, Glawischnig E, et al. Modulation of CYP79 genes and glucosinolate profiles in Arabidopsis by defense signaling pathways. Plant Physiology, 2003, 131(1): 298-308.

[20] Mldrup M E, Geu-Flores F, Halkier B A. Assigning gene function in biosynthetic pathways: Camalexin and beyond. The Plant Cell Online, 2013, 25(2): 360-367.

[21] Pfalz M, Vogel H, Kroymann J. The gene controlling the indole glucosinolate modifier1 quantitative trait locus alters indole glucosinolate structures and aphid resistance in Arabidopsis. The Plant Cell Online, 2009, 21(3): 985-999.

[22] Zhou N, Tootle T L, Glazebrook J. Arabidopsis PAD3 , a gene required for camalexin biosynthesis, encodes a putative cytochrome P450 monooxygenase. The Plant Cell Online, 1999, 11(12): 2419-2428.

[23] Mao G, Meng X, Liu Y, et al. Phosphorylation of a WRKY transcription factor by two pathogen-responsive MAPKs drives phytoalexin biosynthesis in Arabidopsis. The Plant Cell Online, 2011, 23(4): 1639-1653.

[24] Nafisi M, Snderby I E, Hansen B G, et al. Cytochromes P450 in the biosynthesis of glucosinolates and indole alkaloids. Phytochemistry reviews, 2006, 5(2-3): 331-346.

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