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| Cloning and Expression Analysis of CiDHN1 Gene in Caragana intermedia |
| WAN Yong-qing1, YANG Yang1, ZHANG Chun-lin1, WAN Dong-li2, YANG Ai-qin3, YANG Qi1, WANG Rui-gang1, LI Guo-jing1 |
1. College of Life Sciences, Inner Mongolia Agricultural University, Hohhot 010018, China;
2. Grassland Research Institute, Chinese Academy of Agricultural Sciences, Hohhot 010010, China;
3. Inner Mongolia Yijinhuoluo Economic Crops Workstation, Erdos 017200, China |
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Abstract Dehydrins (DHNs; LEA D11 family) are subfamily of group 2 LEA proteins that accumulate to high levels during late stages of seed development and in vegetative tissues subjected to abiotic treatment. A fragment of DHN1 gene was isolated from the suppression subtractive hybridization (SSH) library of Caragana intermedia. The full length sequence of DHN1 was obtained by RACE cloning. Sequence analysis indicated the full length cDNA of CiDHN1 was 1 140 bp, with open reading frame 891bp, deduced encoding 297 amino acids including five Y-segments and one K-segment, the most similarity protein was DHN of Cornus sericea with similarity 39%. CiDHN1 clusters as a unique branch by phylogenetic analysis and was referred as a novel protein with unknown function. Subcellular localization observed CiDHN1 was localized in the plasma membrane and cytoplasm. The transcripts of CiDHN1 were induced under different abiotic stresses, such as cold, dehydration and NaCl. Overexpression of CiDHN1 in Arabidopsis showed susceptible phenotype under 200mmol/L NaCl treatment. The roles of CiDHN1 in Caragana intermedia resist to environmental stresses need further investigation.
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Received: 02 November 2015
Published: 01 December 2015
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