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| Mutagenesis and Mutant Genetic Stability of the Endophytic Fungus Gibberella moniliformis EZG0807 from Curcuma wenyujin |
| YAN Jv-fen, QI Ning-bo, WANG Su-ping, ZHAO Jian-feng, YANG Shu-lin |
| The School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China |
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Abstract In order to obtain a strain with good genetic stability and high activity of secondary metabolites, the wild-type strain Gibberella moniliformis EZG0807 was mutated by a large gradient superconducting magnet (JMT-16T50F) which can simulate micro and hyper-gravity environment. After G. moniliformis EZG0807 was mutated for 24 h, 48 h and 72 h under these conditions, 139 mutants were isolated with the spread plate method. Among them, a highly active mutant M7226 was selected according to the ability of antibacterial and antitumor activity with the filter paper and MTT method, respectively. Furthermore, the biomass of mycelia growth and the bioactivity of secondary metabolites of the strain M7226 within ten generations were investigated to detect its genetic stability. Accordingly, a highly active and stable heritable strain M7226 was got after mutagenesis with the superconducting magnet, which demonstrated that this superconducting magnet can be used as a new method for mutagenesis breeding and laid the foundation for the subsequent separation and purification of secondary metabolites.
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Received: 18 March 2014
Published: 25 May 2014
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[1] 刘录祥, 郑企成. 空间诱变与作物改良. 北京: 原子能出版社, 1997. Zhou L X, Zheng Q C. Space-induced mutations for crop improvement. Beijing: Atomic Energy Press, 1997.
[2] Qian A R, Yin D C, Yang P F, et al. Development of a ground-based simulated experimental platform for gravitational biology. IEEE Transactions on Applied Superconductivity, 2009, 19(2): 42-46.
[3] Qian A R, Yin D C, Yang PF, et al. Application of diamagnetic levitation technology in biological sciences research. IEEE Transactions on Applied Superconductivity, 2013, 23(1): 3600305.
[4] Hill R J, Larkin O J, Dijkstra C E, et al. Effect of magnetically simulated zero-gravity and enhanced gravity on the walk of the common fruitfly. Journal of the Royal Society Interface, 2012, 9(72): 1438-1449
[5] Liu Y M, Zhu D M, Strayer D M, et al. Magnetic levitation of large water droplets and mice. Advances in Space Research, 2010, 45(1): 208-213.
[6] Manzano A I, van Loon J J, Christianen PC, et al. Gravitational and magnetic field variations synergize to cause subtle variations in the global transcriptional state of Arabidopsis in vitro callus cultures. BMC Genomics, 2012, 13(1): 105.
[7] Herranz R, Larkin O J, Dijkstra C E, et al. Microgravity simulation by diamagnetic levitation: effects of a strong gradient magnetic field on the transcriptional profile of Drosophila melanogaster. BMC Genomics, 2012, 13(1): 52.
[8] Coleman C B, Gonzalez-Villalobos R A, Allen PL, et al. Diamagnetic levitation changes growth, cell cycle, and gene expression of Saccharomyces cerevisiae. Biotechnology and Bioengineering, 2007, 98(4): 854-863.
[9] Yan J F, Shang P, Zheng D, et al. Effect of simulated space gravity environment on Gibberella moniliformis EZG0807. Current Microbiology, 2012, 64(5): 469-476.
[10] 朱大诚, 徐彭. 医学功能学科实验指导. 第二版. 北京: 中国协和医科大学出版社, 2010. Zhu D C, Xu P. Manual of medical functional disciplines. 2nd. Beijing: Peking Union Medical College Press, 2010.
[11] Mossman T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assay. Journal of Immunological Methods, 1983, 65(1-2): 55-63.
[12] 高杨, 殷红, 孙宇宏, 等. 产小檗碱内生真菌的诱变. 菌物研究, 2008, 6(4): 216-219. Gao Y, Yin H, Sun Y, et al. Mutagenesis of a berberine-producing endophytic fungus. Journal of Fungal Research, 2008, 6(4): 216-219.
[13] 汪模辉, 邓天龙, 廖梦霞. 含砷金矿的磁场强化生物预氧化. 应用化学, 2000, 17(4): 362-365. Wang M H, Deng T L, Liao M X. Study on magnetization intensified bacterial preoxidation of arsenal gold ores. Chinese Journal of Applied Chemistry, 2000, 17(4): 362-365.
[14] 李国栋. 2003~2004年生物磁学研究和应用的新进展. 生物磁学, 2004, 4(4): 25-26. Li G D. New progrees of research and application of biomagnetism is 2003-2004. Biomagnetism, 2004, 4(4): 25-26.
[15] Koneracka M, Kopcansky P, Antalik M, et al. Immobilization of proteins and enzymes to fine magnetic particles. Journal of Magnetism and Magnetic Materials, 1999, 201(1): 427-430.
[16] Liu M, Gao H, Shang P, et al. Magnetic field is the dominant factor to induce the response of Streptomyces avermitilis in altered gravity simulated by diamagnetic levitation. PLOS One, 2011, 6(10): 1-5.
[17] Okudaa M, Saitoa K, Kamikadoa T, et al. New 7 T superconducting magnet system for bacterial cultivation. Cryogenics, 1995, 35(1): 41-47.
[18] Iwasaka M, Ikehata M, Miyakoshi J, et al. Strong static magnetic field effects on yeast proliferation and distribution. Bioelectrochemistry, 2004, 65: 59-68.
[19] Nakamura K, Okuno K, Ano T, et al. Effect of high magnetic field on the growth of Bacillus subtilis measured in a newly developed superconducting magnet biosystem. Bioelectrochemistry and Bioengergetics, 1997, 43(1): 123-128.
[20] Rosado H, Stapleton PD, Taylor P W. Effect of simulated microgravity on the virulence properties of the opportunistic bacterial pathogen Staphylococcus aureus. AIAA 57th International Astronautical Congress, IAC 2006, 1356-1363.
[21] Lanfang L, Rebecca D, Joan A, et al. Secondary metabolism in Brassica Rapa under hypergravity. 37th COSPAR Scientific Assembly. Montréal, Canada, 2008, 1753.
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