Please wait a minute...

中国生物工程杂志

China Biotechnology
China Biotechnology  2012, Vol. 32 Issue (03): 32-38    DOI:
    
Relationship between SR,α-Tubulin and Resistance to Aspergillus Flavas in Peanut
YAN Hai-yan1, ZONG Cheng-zhi1, ZHAI Gang1, MA Sheng-cai1, SHAN Shi-hua2
1. College of life Sciences, South-Central University for Nationalities, Wuhan 430074, China;
2. Shandong Peanut Research Institute, Qingdao 266100, China
Download: HTML   PDF(1112KB) HTML
Export: BibTeX | EndNote (RIS)      

Abstract  

Signal recognition particle receptor (SR) is important in secretive protein synthesis and secretion process. Tubulin is essential in various life process inside cells. Comparison between proteins encoded by ESTs of α-tubulins and SR in GenBank database of Aspergillus Flavas resistance(C20R) and sensitive(TFR) cultivars reveals that for two types of α-tubulins α and α7, there are 3 ESTs each in TFR early seed developmental stage 5, there is only one EST each in C20R seed developmental stage 5 and 7. For SR, only in C20R seed developmental stage 6 and 7, there is one EST in each stage, none in any seed developmental stage of TFR. QPCR assay on expression of α-tubulins and SR in different organ and developmental stage of peanut fruit of Aspergillus Flavas resistance cultivar KB153 and sensitive cultivar JH1012 indicates that in small fruit stage of fruit early developmental stage, SR and two types of α-tubulins all up-regulated in Aspergillus Flavas resistance cultivar KB153 than sensitive cultivar JH1012. This suggests that protein transport via ER intermediated by SR is related to Aspergillus Flavas resistance. SR is also up-related in cotyledons of Aspergillus Flavas resistance cultivar KB153. This is corresponds to the higher content of storage protein including proteinase inhibitor in Aspergillus Flavas resistance cultivars.



Key wordsAspergillus flavas      Peanut      α-Tubulin      Early fruit developmental stage      SR     
Received: 08 December 2011      Published: 25 March 2012
ZTFLH:  Q5  
Cite this article:

YAN Hai-yan, ZONG Cheng-zhi, ZHAI Gang, MA Sheng-cai, SHAN Shi-hua. Relationship between SR,α-Tubulin and Resistance to Aspergillus Flavas in Peanut. China Biotechnology, 2012, 32(03): 32-38.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2012/V32/I03/32

[1] Xu Z D, Wu Q. Research progress in tubulin. J Anhui Instit Edu, 1999,2(86):73-74.
[2] Zhou H T, Xu L, Zhen W Z, et al. Research on relationship between α-tubulin and cutoplasmic male sterility in Maize. J Xianmen U(Natural Sci), 2003,42(1):107-111.
[3] Zhao J L, Zhao Z J, Zhang H. Relationship between freezing tolerance of root-tip cells and cold stability of microtubules and tubulin in cucumber. J Hebei U(Natural Sci), 2006,26(2):188-192.
[4] Yang Y, Wang B H, Li Z F, et al. The polymerization and denaturation of brain tubulin and the effect of taxol by differential scanning calorimetry. Acta Physico-Chimica Sinica, 1999,15(2):182-185.
[5] Shan S, Yan H, Li C, et al. Primary expression analysis of differential genes in peanut seed capsule with resistance to A. flavus. J Peanut Sci, 2005,34(4): 21-24.
[6] Hegde R S, Kang S. The concept of translocational regulation. J Cell Biol, 2008, 182(2): 225-232.
[7] Powers T, Walter D P. Co-translational protein targeting catalyzed by the Escherichia coli signal recognition particle and its receptor. EMBO J, 1997, 16(16): 4880-4886.
[8] Mandon E C, Jiang Y, Gilmore R. Dual recognition of the ribosome and the signal recognition particle by the SRP receptor during protein targeting to the endoplasmic reticulum. J Cell Biol, 2003, 162(4): 575-585.
[9] Herskovits A A, Seluanov A, Rajsbaum R, et al. Evidence for coupling of membrane targeting and function of the signal recognition particle (SRP) receptor FtsY. EMBO Report, 2001, 2(11): 1040-1046.
[10] Jiang Y, Cheng Z, Mandon E C, et al. An interaction between the SRP receptor and the translocon is critical during co-translational protein translocation. J Cell Biol, 2008, 180(6): 1149-1161.
[11] Zhang X, Kung S, Shan S. Demonstration of a multi-step mechanism for assembly of the SRP-SRP Receptor complex: implications for the catalytic role of SRP RNA. Journal of Mol Biol, 2008, 381(3): 581-593.
[12] Young J C, Andrews D W. The Signal recognition particle receptor alpha subunit assembles co-translationally on the endoplasmic reticulum membrane during an mRNA-encoded translation pause in vitro. EMBO J, 1996, 15(1): 172-181.
[13] Bürk J, Weiche B, Wenk M, et al. Depletion of the signal recognition particle receptor inactivates ribosomes in Escherichia coli. J Bacteriol, 2009, 191(22): 7017-7026.
[14] Ogg S C, Poritz M A, Walter P. Signal Recognition Particle receptor is important for cell growth and protein secretion in Saccharomyces cerevisiae. Mol Biol Cell, 1992, 3(8): 895-911.
[15] Maruyama N, Mun L C, Tatsuhara M, et al. Multiple vacuolar sorting determinants exist in soybean 11S globulin. Plant Cell, 2006,18(5): 1253-1273.
[16] Zong C. Study of Aspergillus flavus resistance genes in developing peanut. Wuhan: South-Central University for Nationalities, College of life sciences, 2010.
[17] Yan H Y, Ding Y, Wu Y X. Laboratory manual for molecular biology and genomic engineering. Wuhan: Wuhan University Publisher, 2009.18-41.
[18] Yan H Y, Zong C Z, Bao W Z, et al. Relationship between peanut Aspergillus flavus resistance and glycogen synthase kinase-3. Acta Agriculturae Boreali-sinica,2011,26 (5):1-4.
[19] Yan H Y, Zong C Z, Jing C H, et al. Analysis of relativity of receptor-like kinase to Aspergillus flavus resistance in peanut. Acta Agriculturae Boreali-sinica,2011,26 (4):198-201.
[20] Ji R C, Tang Z X, Li G X, et al. Determination of resistance to Aspergillus flavus and productivity of five peanut cultivars. Fujian Agri Sci Technol, 2000,(3):10-11.
[21] Zhou G Y, Liang X Q, Li Y C, et al. Evaluation and application of introduced peanut cultivars for resistance to Aspergillus flavus. J Peanut Sci, 2002,31(2):14-17.
[22] Liang X, Zhou G, Pan R. Wax and curticle of peanut seed coat in relation to infection and aflatoxin production by Aspergillus flavus. J Trop Subtrop Bot, 2003, 11(1): 11-14.
[23] Yan H Y, Zong C Z, Ma G H,et al. Relationship between ribosome protein L41 and resistance to Aspergillus flavus. Acta Agriculturae Boreali-sinica,2011,26(6):16-19.
[24] Scales T M E, Lin S, Kraus M, et al. Nonprimed and DYRK1A-primed GSK3β-phosphorylation sites on MAP1B regulate microtubule dynamics in growing axons. J Cell Sci, 2009, 122(14): 2424-2435.
[25] Wang S, Huang J, He J, et al. RPL41, a small ribosomal peptide deregulated in tumors, is essential for mitosis and centrosome integrity. Neoplasia, 2010,12(3): 284-293.
[26] Ferralli J, Ashby J, Fasler M, et al. Disruption of microtubule organization and centrosome function by expression of tobacco mosaic virus movement protein. J Virol, 2006,80(12): 5807-5821.
[27] Morrissette N S, Mitra A, Sept D, et al. Dinitroanilines bind α-tubulin to disrupt microtubules. Mol Biol of the Cell, 2004,15(4):1960-1968.
[28] Ma C, Li C, Ganesan L, et al. Mutations in α-tubulin confer dinitroaniline resistance at a cost to microtubule function. Molecular Biol Cell, 2007, 18(12):4711-4720.
[29] Migliaccio G, Nicchitta C V, Blobel G. The signal sequence receptor, unlike the Signal Recognition Particle receptor, is not essential for protein translocation. J Cell Biol, 1992, 117(1): 15-25.
[30] Friedman J R, Webster B M, Mastronarde D N, et al. ER sliding dynamics and ER-mitochondrial contacts occur on acetylated microtubules. J Cell Biol, 2010,190(3): 363-375.
[31] Liang X Q, Pan R Z, Bin J H. Research progress in mechanism of A. flavus resistance of peanut. Chinese J Oil Crop Sci, 2000,22(3):77-80.
[32] Zhou G, Liang X, Li Y, et al. Comparative study on seed coat ultrastructure of resistant and susceptible varieties to Aspergillus flavas in peanut. Chinese J of Oil Crop, 1999,17(1):43-53.
[33] Guo B, Chen X, Dang P, et al. Peanut gene expression profiling in developing seeds at different reproduction stages during Aspergillus parasiticus infection. BMC Dev Biol, 2008, 8(2): 12-27.
[34] Liang X, Pan R, Zhou G. Relation of trypsin inhibitor in peanut seed and resistance to Aspergillus Flavus invasion. Acta Agro Sinica, 2003, 29(2): 295-299.

[1] WANG You-bei,GUO Si-yu,CHANG Bi-bo,YE Rui-fang,HUA Qiang. Establishment of Conjugation System for the Spiramycin Producer Streptomyces spiramyceticus[J]. China Biotechnology, 2021, 41(2/3): 45-52.
[2] GUO Jing,HOU Zhan-ming. Folpcs1 Is Responsible for Asexual Reproduction and Vegetative Growth in Fusarium oxysporum f. sp. Lini.[J]. China Biotechnology, 2020, 40(3): 48-64.
[3] TANG Xin,MAO Xin-fang,MA Bin-yun,GOU Ping. Antimicrobial Peptides: Current Status and Future Challenges[J]. China Biotechnology, 2019, 39(8): 86-94.
[4] Yue ZHAO,Hao WU,Jian-jun QIAO. Research on the Regulatory Mechanisms of Bacterial Cell Wall Growth[J]. China Biotechnology, 2018, 38(8): 92-99.
[5] Zhong-yang YE,Huai-yu QIU,Bing-hua ZHU,Ze LI,Ye ZHU,Li-gui WANG. Research Progress of sRNA Regulates the Expression of Genes in Related with Bacterial Resistance[J]. China Biotechnology, 2018, 38(7): 89-93.
[6] Yu-rong ZHENG,Jun-bao JIN,Xin-nian WU,Guang-zu BAI,Qiu-yan LIU. Study on Disruptive Technology of Cellulose Biodegradation ased on Multi-data Sources[J]. China Biotechnology, 2018, 38(5): 92-103.
[7] ZHANG Li-li, XU Bi-yu, LIU Ju-hua, JIA Cai-hong, ZHANG Jian-bin, JIN Zhi-qiang. Analysis of Banana MaASR1 Gene Expression Profiles in Arabidopsis Under Drought Stress[J]. China Biotechnology, 2017, 37(11): 59-73.
[8] HOU Bing-xiao, LIU Shan-na, WANG Bin-bin, ZHU Hong-ji, QIAO Jian-jun. Advances on Regulatory Mechanism of Heat-shock Proteins[J]. China Biotechnology, 2016, 36(9): 87-93.
[9] BAI Li-ping, JIANG Rong, GUO Lian-hong, ZHANG Yang, LI Yuan. The Effects of ste3 and ste4 Genes Double Disruption in Ebosin Biosynthesis[J]. China Biotechnology, 2015, 35(11): 23-28.
[10] LIU Wei, ZHENG Pu, JIN Xin-na. Effects of Disrupting Acetate Formation Pathways in Corynebacterium acetoacidophilum on Succinate Production Under Oxygen Deprivation[J]. China Biotechnology, 2014, 34(9): 48-55.
[11] ZHU Yi-long, LI Chang, GUO Yan, LIU Cun-xia, DU Shou-wen, WANG Mao-peng, JIN Ning-yi. Construction and Selection of the Recombinant Fowlpox Expressing HIV-1 gag[J]. China Biotechnology, 2014, 34(1): 57-63.
[12] YAO Yuan-feng, ZHAO Ying, ZHAO Guang-rong. Artificial sRNAs Silencing csrA to Optimize the Production of L-tyrosine in Escherichia coli[J]. China Biotechnology, 2013, 33(8): 61-66.
[13] ZHANG Cai-bo, ZHANG Yan-hua, LIU He-yang, WANG Han-yu, ZENG Wen-bing, RONG Ting-zhao, CAO Mo-ju. Construction of Fingerprinting Using SRAP and SSR Markers for Maize Inbred Lines by Space Flight[J]. China Biotechnology, 2013, 33(10): 103-110.
[14] ZHU Kuan-peng, ZHAO Shu-jin. Double-stranded RNA-mediated Gene Silencing and Over-expression of FmSTS in Polygonum multiflorum Thunb Hairy Roots[J]. China Biotechnology, 2012, 32(08): 41-48.
[15] E Guang-xin, LIU Di, ZHANG Dong-jie, CUI Yu. Cloning, Expression and Polymorphism Analysis of Porcine SRPK3 Gene[J]. China Biotechnology, 2011, 31(03): 46-54.