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| Analysis of the Bacterial Community in Gastric Samples from Mice with TypeⅡDiabetes by Using PCR-DGGE Fingerprint |
| DU Cai-he1, HU Fang2, WEI Ting-ting1, ZHANG Ren-min1, ZHANG Hong-lin2, ZHOU Dong-rui1, LU Zu-hong1,2 |
1. Research Center for Learning Science, Southeast University, Nanjing 210096, China;
2. School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China |
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Abstract Gastrointestinal complications are common in patients with diabetes mellitus which involve the acute gastritis and gastric ulcer. Because gastric dyskinesis, delayed gastric emptying, gastric bacterial over-growth, these will further lead to intestinal diseases. Research on the change in diabetes stomach content bacterium group structure, and it’is important for the study of diabetes pathogenesis and the treatment of complications. Bacterial DNAs from 10 type 2 diabetes mice and 10 normal control mice were extracted from gastric contents and gastric mucosal samples and then characterized by PCR-denaturing gradient gel electrophoresis (DGGE). After DGGE profilings was obtained,the diversity and similarity analyses were carried out by the number of band,Shannon-Weaver(H’), Margalef index(R), Pielou index(E) and Simpson index(D),cluster analysis and cloning sequencing. The results show that there was no significant difference in Shannon-Weaver diversity index、Margalef index 、Pielou index、and Simpson index between experimental group and control group. There is no significant distinction in the Dice similarity coefficient (Cs) between the two groups. The result of sequencing illustrates that Lactobacillus is in the gastric of normal control mice, but type 2 diabetes mice. These results suggest that lactobacillus is closely related to type 2 diabetes.
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Received: 03 August 2011
Published: 25 March 2012
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