| 研究报告 |
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| The SARS-CoV 3a and 7a protein may enhance the induction of IFN-β |
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Abstract 3a and 7a are nonstructural proteins of SARS-CoV, which are encoded separately by ORF 3a and ORF 7a in SARS-CoV genome. The expression of 3a has been founded in cells infected by virus in vivo or in vitro. Firstly, the pGL3-Control vector was reconstructed , the pGL3-Enhancer vector deletious of SV40 promoter gene was obtained . Then the IFN-β promoter gene was cloned into the pGL3-Enhancer vector and pGL-IP21, the Luciferase reporter plasmid with IFN-β promoter was OK. The availability of pGL-IP21 was verified by NDV ,the inductor of IFN-β, the Luciferase activity was assayed in cells transfected with pGL-IP21 by Luminometer. In order to see the function of 3a and 7a protein of SARS-CoV，CHO cells expressing 3a or 7a protein were transfected with pGL-IP21, the intensity of luciferase activity was analyzed . By analysis, in vitro, 3a and 7a protein of SARS-CoV have the similar ability in triggering the expression of Luceferase gene, i.e 3a and 7a protein of SARS-CoV can effectively activate the promoter fragment of IFN-β gene. This result will help studying the function of 3a and 7a protein and provide a method to study the nosogenesis mechanism of SARS-CoV.
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Received: 06 July 2006
Published: 25 December 2006
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