|
|
|
| Effects of Chitosan Oligosaccharides Attenuating Menadione-induced Injury in Macrophages |
| MA Pan1,2, LIU Hong-tao1,2, XU Qing-song1, BAI Xue-fang1, DU Yu-guang1 |
1. Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China;
2. Graduate School of Chinese Academy of Sciences, Beijing 100049, China |
|
|
|
Abstract Aim: To investigate the effects of Chitosan oligosaccharides (COS) on attenuating menadione-induced stress injury in macrophages. Method: MTT assay was used to examine the cell viability, and the reagent kits were used to investigate the activities of enzymes and the products of lipid peroxidation in redox system. Results: By using MTT assay and detecting the activities of LDH (lactate dehydrogenase) in the cultural supernatants, it was confirmed that chitosan oligosaccharides could attenuate menadione-induced stress injury in macrophages. Moreover, the results showed that COS could also restore activities of endogenous antioxidants including superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX), and suppressing the production of lipid peroxidation. Those results could explain the mechanisms of COS on attenuating menadione-induced stress injury in macrophages. Conclusion: Stress injury induced by menadione in macrophages could be blocked by COS.
|
|
Received: 20 December 2010
Published: 28 June 2011
|
|
|
|
|
| [1] Jomova K, Valko. Advances in metal-induced oxidative stress and human disease. Toxicology, 2011, 283(2-3):65-87.
[2] del Hoyo P, Garcia-Redondo A, de Bustos F, et al. Oxidative stress in skin fibroblasts cultures from patients with Parkinson's disease. BMC Neurol, 2010, 10: 95-101.
[3] Li L, Li W, Jung S W, et al. Protective effects of decursin and decursinol angelate against amyloid beta-protein-induced oxidative stress in the PC12 cell line: the role of Nrf2 and antioxidant enzymes. Biosci Biotechnol Biochem, 2011, 75(3): 434-442.
[4] Lee S, Park Y, Zuidema M Y, et al. Effects of interventions on oxidative stress and inflammation of cardiovascular diseases. World J Cardiol, 2011, 3(1): 18-24.
[5] Park J K, Chung M J, Choi H N, et al. Effects of the molecular weight and the degree of deacetylation of chitosan oligosaccharides on antitumor activity. Int J Mol Sci, 2011, 12(1): 266-277.
[6] Pan M, Hong-Tao L, Peng W, et al. Chitosan oligosaccharides inhibit LPS-induced over-expression of IL-6 and TNF-α in RAW264.7 macrophage cells through blockade of mitogen-activated protein kinase (MAPK) and PI3K/Akt signaling pathways. Carbohydr Polym, 2011, 84(4): 1391-1398.
[7] Fernandes J C, Tavaria F K, Fonseca S C, et al. In vitro screening for anti-microbial activity of chitosans and chitooligosaccharides, aiming at potential uses in functional textiles. J Microbiol Biotechnol, 2010, 20(1): 311-318.
[8] Yuan W P, Liu B, Liu C H, et al. Antioxidant activity of chito-oligosaccharides on pancreatic islet cells in streptozotocin-induced diabetes in rats. World J Gastroenterol, 2009, 15(11): 1339-1345.
[9] Liu H T, Li W M, Xu G, et al. Chitosan oligosaccharides attenuate hydrogen peroxide-induced stress injury in human umbilical vein endothelial cells. Pharmacol Res, 2009, 59(3): 167-175.
[10] Xu Q, Ma P, Yu W, et al. Chitooligosaccharides protect human embryonic hepatocytes against oxidative stress induced by hydrogen peroxide. Mar Biotechnol, 2010, 12(3): 292-298.
[11] Qiao Y, Bai X F, Du Y G. Chitosan oligosaccharides protect mice from LPS challenge by attenuation of inflammation and oxidative stress. International Immunopharmacology, 2011, 11(1): 121-127.
[12] Michael M S, John J G, Joseph D P. Regulation of macrophage inflammatory protein-1α mRNA by oxidantive stress. J Biol Chem, 1996, 271: 5878-5883.
[13] Gerasimenko J V, Gerasimenko O V, Palejwala A, et al. Menadione-induced apoptosis: roles of cytosolic Ca(2+) elevations and the mitochondrial permeability transition pore. J Cell Sci, 2002, 115: 485-497.
[14] Leist M, Single B, Castoldi A F, et al. Intracellular adenosine triphosphate (ATP) concentration: a switch in the decision between apoptosis and necrosis. J Exp Med, 1997, 185: 1481-1486.
[15] Bowie A G, O'Neill L A. Vitamin C inhibits NF-kappa B activation by TNF via the activation of p38 mitogen-activated protein kinase. J Immunol, 2000, 165:7180-7188.
[16] Paula M B, John R H, Virginia L S. Oxidant-mediated inhibition of ligand uptake by the macrophage mannose receptor. J Biol Chem, 1988, 263:1240-1247. |
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
