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| S100A6 Promotes Cell Proliferation of Colorectal Cancer via Upregulating IL-6 Expression of Macrophages |
Lu CHEN,Mao HUANG,Qi PENG,Jia-li ZHAO,Jia-qing XIE,Lu LIN,Li-jun HU,Yi-yun HUANG,Qin HU,Lan ZHOU( ) |
| Key Laboratory of Laboratory Medical Diagnostics of Ministry of Education, Chongqing Medical University, Chongqing 400016, China |
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Abstract Objective: To explore whether Calcyclin S100A6 in tumor microenvironment promotes cell proliferation of colorectal cancer(CRC) through affecting macrophages (Mφ) and its mechanism. Methods: Prokaryotic expression was used to prepare recombinant human protein GST-S100A6 (rS100A6) and GST (as control). THP-1 were induced to Mφ by PMA (Phorbol-12-myristate-13-acetate). A6-Mφ were the macrophages which were treated with rS100A6 for 24h. The proliferation of CRC HCT116 cells was detected by Trypan blue staining, CCK8 and crystal violet staining. IL-6 mRNA and protein level in A6-Mφ were tested with quantitative polymerase chain reaction (qPCR) and Western blot, respectively. The protein levels of total JAK2 and STAT3 (t-JAK2 and t-STAT3) and the phosphorylated JAK2 and STAT3 (p-JAK2 and p-STAT3) in HCT116 cells were detected by Western blot. Results: (1) rS100A6 and GST were prepared successfully. (2) A6-Mφ promoted proliferation of HCT116 cells (P<0.05). (3) rS100A6 upregulated IL-6 expression in macrophages (P<0.05). (4) IL-6R blocking antibody partly reversed the facilitation of A6-Mφ to proliferation of HCT116 cells (P<0.05). (5) A6-Mφ increased protein levels of p-JAK2 and p-STAT3 in HCT116 cells (P<0.05), but not t-JAK2 and t-STAT3. Conclusion: S100A6 in tumor microenvironment facilitates proliferation of HCT116 cells through upregulating IL-6 expression in macrophages and activating IL-6/JAK2/STAT3 pathway in HCT116 cells.
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Received: 06 September 2018
Published: 08 May 2019
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Corresponding Authors:
Lan ZHOU
E-mail: zhoulan0111@foxmail.com
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Cite this article:
Lu CHEN,Mao HUANG,Qi PENG,Jia-li ZHAO,Jia-qing XIE,Lu LIN,Li-jun HU,Yi-yun HUANG,Qin HU,Lan ZHOU. S100A6 Promotes Cell Proliferation of Colorectal Cancer via Upregulating IL-6 Expression of Macrophages. China Biotechnology, 2019, 39(4): 1-7.
URL:
https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20190401 OR https://manu60.magtech.com.cn/biotech/Y2019/V39/I4/1
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