Analysis of Differential lncRNA Expression Profile in the Macrophages after <i>Mycobacterium tuberculosis</i> Stimulation

doi:10.13523/j.cb.20180501

China Biotechnology

2018, Vol. 38

Issue (5): 1-9 DOI: 10.13523/j.cb.20180501

Analysis of Differential lncRNA Expression Profile in the Macrophages after Mycobacterium tuberculosis Stimulation

Yang TAN,Sheng LIU,Feng-ling LUO,Xiao-lian ZHANG(

)

Department of Immunology, State Key Laboratory of Virology, Hubei Province Key Laboratory of Allergy and Immunology and Institute of Medical Research, Wuhan University School of Medicine,Wuhan 430071, China

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Abstract

To screen for differentially lncRNAs expression profile and predict their functional roles in the macrophage after Mycobacterium tuberculosis (M.tb) stimulation. Firstly, microarray and bioinformatics analysis of lncRNA and mRNA expression profiles in RAW264.7 macrophage after stimulation with M.tb for 24h.Then, 16 differentially expressed lncRNAs from microarray analysis were further verified by RT-qPCR using H37Rv infected macrophages and mouse model. The results shown that the expression levels of 4 730 lncRNAs was up-regulated, and the expression levels of 9 558 lncRNAs was down-regulated. 16 differentially expressed lncRNAs from microarray screening were associated with protein coding genes in adjacent locations. The mRNA function annotation analysis revealed that the mRNAs of differential expression were mainly concentrated in the biochemical process of transcriptional regulation, phosphorylation, apoptosis and MAPK signaling pathway which participating in the biochemical process of anti-tuberculosis. The expression trend of 4 lncRNAs in the iH37Rv stimulated RAW264.7 and mouse infection model was vertified as the same in both microarray and RT-qPCR analysis. Three of these 4 lncRNAs was up-regulated and one of them was down-regulated.The abnormal expression of lncRNAs may provide clues to the dysfunction of macrophages with M.tb infection, and further research will focus on the investigation of the function and regulation mechanism of lncRNA in M.tb infected macrophage.

Key words： Mycobacterium tuberculosis (M.tb) Macrophage Long non-coding RNA (lncRNA) mRNA Microarray analysis

Received: 19 January 2018 Published: 05 June 2018

ZTFLH:

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Corresponding Authors: Xiao-lian ZHANG E-mail: zhangxiaolian@whu.edu.cn

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	Yang TAN
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	Xiao-lian ZHANG

Cite this article:

Yang TAN,Sheng LIU,Feng-ling LUO,Xiao-lian ZHANG. Analysis of Differential lncRNA Expression Profile in the Macrophages after Mycobacterium tuberculosis Stimulation. China Biotechnology, 2018, 38(5): 1-9.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20180501 OR https://manu60.magtech.com.cn/biotech/Y2018/V38/I5/1

Table 1 Primers of 16 differentially expressed lncRNAs for RT-qPCR

Fig.1 Differential expression profile of 200 lncRNAs in RAW264.7 cells after iH37Rv stimulation for 24h C1,C2 represent for untreated group,T1 represents for iH37Rv stimulation group

Table 2 Differential expression of 16 lncRNAs, and their positional relationship with adjacent protein-coding genes

Fig.2 Function annotation of differentially expressed mRNAs (a)-(c)GO analysis (d) KEGG analysis

Fig.3 Differential expression levels of 16 lncRNAs in RAW264.7 cells after iH37Rv stimulation for 24h by RT-qPCR Numbers 1 to 16 represent for lncRNAs as shown in Table 1 (* P<0.05,vs. untreated control group)

Fig.4 Differential expression levels of 16 lncRNAs in mouse lung (a) and spleen (b)tissues after H37Rv infection for 7 days by RT-qPCR * P<0.05,vs. untreated control group


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