<em>Chlorococcum</em> sp.混养过程碳氮调控对累积类胡萝卜素和脂肪酸成分影响研究

doi:10.13523/j.cb.20150705

中国生物工程杂志

2015, Vol. 35

Issue (7): 30-36 DOI: 10.13523/j.cb.20150705

研究报告

Chlorococcum sp.混养过程碳氮调控对累积类胡萝卜素和脂肪酸成分影响研究

柴鹏^1,2, 丰平仲¹, 王学伟³, 王忠铭¹, 李谢昆^1,2, 袁振宏¹

1. 中国科学院广州能源研究所中国科学院可再生能源重点实验室广州 510640;
2. 中国科学院大学北京 100039;
3. 中国科学院广州能源研究所佛山三水能源环境技术创新与育成中心佛山 528137

Effect of Carbon and Nitrogen Combination on the Cartenoid and Fatty Acids Accumulation of Chlorococcum sp. under Mixotrophic Cultivation

CHAI Peng^1,2, FENG Ping-zhong¹, WANG Xue-wei³, WANG Zhong-ming¹, LI Xie-kun^1,2, YUAN Zhen-hong¹

1. Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China;
2. University of Chinese Academy of Sciences, Beijing 100039, China;
3. Foshan Innovation and Incubation Center of Energy and Environmental Technology, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Foshan 528137, China

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摘要：

在BG-11培养液的基础上,选择不同的乙酸钠和硝酸钠浓度组合培养绿球藻Chlorococcum sp.,分析培养过程中干重、类胡萝卜素、总脂及脂肪酸成分,完成Chlorococcum sp.生长及油脂积累的评价。实验结果表明,外源碳源乙酸钠对Chlorococcum sp.的生长和油脂积累有很好的促进作用。乙酸钠和硝酸钠浓度分别为2g/L和1g/L条件下,Chlorococcum sp.培养16天后生物质产率高达276.19mg/(L·d), 同时类胡萝卜素和总脂的产率也分别高达1.347mg/(L·d)和108.59mg/(L·d), 高附加值脂肪酸二十碳五烯酸(EPA)与二十二碳六烯酸(DHA)占脂肪酸总量也超过了1.5%。

关键词： 绿球藻; 混养; 干重; 类胡萝卜素; 脂肪酸

Abstract:

The object was to analyze the effects of different combinations of additional carbon and nitrogen resources on the growth and lipid accumulation of Chlorococcum sp. under modified BG-11 culture pretreated by different concentrations of sodium acetate and sodium nitrate. The biomass of Chlorococcum sp. was determined by dry weight method. The features of lipid accumulation were analyzed by lipid productivity, carotenoid productivity and fatty acid methyl ester (FAME) composition. The results showed that Chlorococcum sp. cultivated in the modified BG11 with mass concentrations of sodium acetate 2g/L and sodium nitrate 1g/L had significantly improved algal growth and lipid accumulation. The final biomass, carotenoid and lipid productivity reached 276.19mg/(L·d), 1.347mg/(L·d) and 108.59mg/(L·d), respectively after 16 days' cultivation. It is worth to note that the yield of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) reached 1.58% of total fatty acids.

Key words: Chlorococcum sp. Mixotrophic cultivation Dry weight Cartenoid Fatty acids

收稿日期: 2015-03-12 出版日期: 2015-07-25

ZTFLH:

TQ645.6

基金资助:

广东省中国科学院全面战略合作项目(2011B090300063),佛山市院市合作项目(2012HY100415) 资助项目

通讯作者: 王忠铭 E-mail: wangzm@ms.giec.ac.cn

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引用本文:

柴鹏, 丰平仲, 王学伟, 王忠铭, 李谢昆, 袁振宏. Chlorococcum sp.混养过程碳氮调控对累积类胡萝卜素和脂肪酸成分影响研究[J]. 中国生物工程杂志, 2015, 35(7): 30-36.

CHAI Peng, FENG Ping-zhong, WANG Xue-wei, WANG Zhong-ming, LI Xie-kun, YUAN Zhen-hong. Effect of Carbon and Nitrogen Combination on the Cartenoid and Fatty Acids Accumulation of Chlorococcum sp. under Mixotrophic Cultivation. China Biotechnology, 2015, 35(7): 30-36.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20150705 或 https://manu60.magtech.com.cn/biotech/CN/Y2015/V35/I7/30

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