技术与方法 |
|
|
|
|
无标签重组人硫氧还蛋白的大规模表达、纯化及活性检测 |
郭云萍1, 孙璐1, 张立剑1, 王增禄2, 高超1, 杨强1, 刘毅1, 张英起2, 屈延1, 陶凌1 |
1. 第四军医大学西京医院 西安 710032; 2. 第四军医大学药学系生物技术中心 西安 710032 |
|
Expression, Purification and Characterization of Non-taged Recombinant Human Thioredoxin |
GUO Yun-ping1, SUN Lu1, ZHANG Li-jian1, WANG Zeng-lu2, GAO Chao1, YANG Qiang1, LIU Yi1, ZHANG Ying-qi2, QU Yan1, TAO Ling 1 |
1. Department of Cardiology, XiJing Hospital, The Fourth Military Medical University, Xi’an 710032, China; 2. Biotechnology Center, School of Pharmacy, The Fourth Military Medical University, Xi’an 710032, China |
引用本文:
郭云萍, 孙璐, 张立剑, 王增禄, 高超, 杨强, 刘毅, 张英起, 屈延, 陶凌. 无标签重组人硫氧还蛋白的大规模表达、纯化及活性检测[J]. 中国生物工程杂志, 2012, 32(08): 62-67.
GUO Yun-ping, SUN Lu, ZHANG Li-jian, WANG Zeng-lu, GAO Chao, YANG Qiang, LIU Yi, ZHANG Ying-qi, QU Yan, TAO Ling. Expression, Purification and Characterization of Non-taged Recombinant Human Thioredoxin. China Biotechnology, 2012, 32(08): 62-67.
链接本文:
https://manu60.magtech.com.cn/biotech/CN/
或
https://manu60.magtech.com.cn/biotech/CN/Y2012/V32/I08/62
|
[1] Ahsan M K, Lekli I, Ray D, et al. Redox regulation of cell survival by the thioredoxin superfamily: an implication of redox gene therapy in the heart.Antioxid Redox Signal,2009,11(11):2141-2158. [2] Burke-Gaffney A, Callister M E, Nakamura H. Thioredoxin:friend or foe in human disease? Trends in Pharmacological Sciences,2005,26(8):398-404. [3] Shioji K, Nakamura H, Masutani H, et al. Redox regulation by thioredoxin in cardiovascular diseases. Antioxidants﹠Redox Signaling,2003,5(6):795-802. [4] Yamawaki H, Haendeler J, BerK B C. Thioredoxin: a key regulator of cardiovascular homeostasis. Circulation Research,2003,93(11):1092-1033. [5] Tao L, Gao E, Bryan N S, et al. Cardioprotective effects of thioredoxin in myocardial ischemia and reperfusion : role of S-nitrosation . Proceedings of the National Academy of Sciences of the United States of Amerca,2004,101(31):11471-11476. [6] Yamawaki H, BerK B C. Thioredoxin:a multifunctional antioxidant enzyme in kidney, heart and vessels. Current Opinion in Nephrology and Hypertension,2005,14(2):149-153. [7] Berndt C, Lillig C H, Holmgren A. Thiol-based mechanisms of the thioredoxin and glutaredoxin systems:implications for diseases in the cardiovascular system. American Journal of Physiology, 2007,292(3):1227-1236. [8] Ago T, Sadoshima J. Thioredoxin and ventricular remodeling. Journal of Molecular and Cellular Cardiology,2006,41(5):762-773. [9] Nakamura H. Thioredoxin and its related molecules:update 2005. Antioxidants﹠Redox Signaling,2005,7(5-6):823-828. [10] Anna D F, Rosa M, Emilia P, et al, High-level expression of Aliciclobacillus acidocaldarius thioredoxin in Pichia pastoris and Bacillus subtilis.Protein Expr Purif, 2003,30:179-184. [11] Khalilzadeh R, Shojaosadati SA, Bahrami A, et al. Over-expression of recombinant human interferon-gamma in high cell density fermentation of Escherichia coli. Biotechnol Lett,2003,25(23):1989-1992. [12] Tabandeh F, Shojaosadati S A, Zomorodipour A, et al.Heat-induced production of human growth hormone by high cell density cultivation of recombinant Escherichia coli. Biotechnol Lett,2004,26(3):245-249. [13] Wollman E E, d’Auriol L, Rimsky L, et al. Cloning and expression of a cDNA for human thioredoxin. Journal of Biological Chemistry,1988,263(30):15506-15512. [14] Miranda-Vizuete A, Damdimopoulos A E, Gustafsson J,et al.Cloning,expression and characterzation of a novel Escherichia coli thioredoxin.Journal of Biological Chemistry,1997,272(49):30841-30847. [15] Reckenfelderbaumer N, Ludemann H, Schmidt H, et al. Identification and functional characterization of thioredoxin from Trypanosoma brucei. Journal of Biological Chemistry,2000,275(11):7547-7552. |
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
|
Shared |
|
|
|
|
|
Discussed |
|
|
|
|