Please wait a minute...

中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志  2015, Vol. 35 Issue (4): 98-106    DOI: 10.13523/j.cb.20150415
综述     
植物内生放线菌功能及生物活性物质研究进展
冯天祥, 王玲, 陈海敏, 盛清, 左锐, 谢文杰
浙江理工大学生命科学学院 杭州 310018
Research Advances on Function and Bioactive Substances of Endophytic actinomycetes
FENG Tian-xiang, WANG Ling, CHEN Hai-min, SHENG Qing, ZUO Rui, XIE Wen-jie
College of Life Sciences, Zhejiang SCI-TECH University, Hangzhou 310018, China
 全文: PDF(644 KB)   HTML
摘要:

植物内生放线菌是与宿主植物存在互利共生关系的一类特殊微生物,具有丰富的生物学功能,能产生许多具有应用潜力的生物活性物质。阐述了植物内生放线菌与宿主植物之间的关系,并从提高宿主植物抗逆性、促进生长和生物固氮作用三个方面总结了植物内生放线菌的生物学作用研究进展;综述了植物内生放线菌产生的抗生素、酶和抗肿瘤活性物质以及这些物质在农业、医药以及食品行业中的应用前景。最后就内生放线菌生物学作用及活性物质研究中存在的问题和发展趋势进行了展望。

关键词: 内生放线菌宿主植物生物学功能生物活性物质    
Abstract:

Endophytic actinomycetes are special groups of microorganisms,associated with the host plant with symbiotic relationship, which have amazing variety of biological function, and can produce lots of potentially biological active substances. The relationship between the Endophytic actinomycetes and the host plant was described, and then the progress in their biological effects on improvement of plant stress resistance, promotion of growth and biological nitrogen fixation were introduced. The antibiotics, enzymes and anti-tumor activity of bioactive substances produced by Endophytic actinomycetes, and the future prospects of these substances in agriculture, medicine and food industry were summarized. Finally, the problems and trends about the biological effects of Endophytic actinomycetes and their bioactive substances were also analyzed.

Key words: Endophytic actinomycetes    Host plants biological functions    Biological active substances
收稿日期: 2014-09-18 出版日期: 2015-04-25
ZTFLH:  Q939  
基金资助:

2014年国家级大学生创新创业训练计划项目(201410338016)、2013年浙江省大学生科技创新项目(2013R406015)、浙江省生物学重中之重(一级)学科资助项目

通讯作者: 陈海敏     E-mail: chenhm@zstu.edu.cn
服务  
把本文推荐给朋友
加入引用管理器
E-mail Alert
RSS
作者相关文章  

引用本文:

冯天祥, 王玲, 陈海敏, 盛清, 左锐, 谢文杰. 植物内生放线菌功能及生物活性物质研究进展[J]. 中国生物工程杂志, 2015, 35(4): 98-106.

FENG Tian-xiang, WANG Ling, CHEN Hai-min, SHENG Qing, ZUO Rui, XIE Wen-jie. Research Advances on Function and Bioactive Substances of Endophytic actinomycetes. China Biotechnology, 2015, 35(4): 98-106.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20150415        https://manu60.magtech.com.cn/biotech/CN/Y2015/V35/I4/98


[1] Stone J K, Bacon C W, White J. An overview of endophytic microbes: Endophytism defined. Microbial Endophytes, 2000, 3:29-33.

[2] Stierle A, Strobel G. Taxol and taxane production by Taxomyces andreanae an endophytic fungus of Pacific yew. Science, 1993, 260(5105): 214.

[3] 李霞, 刘佳佳, 陈建华, 等. 产喜树碱喜树内生真菌的筛选及喜树内生真菌的SRAP分析. 中国生物工程杂志, 2011, 31(7): 60-64. Li X, Liu J J, Chen J H, et al. Screening of camptothecin prodution and SRAP analysis of endophytic fungi from Camptotheca acuminata decne. China Biotechnology, 2011, 31(7): 60-64.

[4] Liu X, Dong M, Chen X, et al. Antioxidant activity and phenolics of an Endophytic xylaria sp. from Ginkgo biloba. Food Chemistry, 2007, 105(2): 548-554.

[5] 张晓晗, 竺锡武, 谭济才, 等. 一株产凝集素的三叶半夏内生真菌的研究. 中国生物工程杂志, 2009, 29(6): 63-67. Zhang X H, Zhu X W, Tan J C, et al. Study on one strain endophytic fungi isolated from Pinellia ternata (Thunb.)Breit which could produce lectins. China Biotechnology, 2009, 29(6): 63-67.

[6] 石晶盈, 陈维信, 刘爱媛. 植物内生菌及其防治植物病害的研究进展. 生态学报, 2006, 26(7): 2395-2401. Shi J Y, Chen W X, Liu A Y. Advances in the study of endophytes and their effects on control of plant diseases. Acta Ecologica Sinica, 2006, 26 (7): 2395-2401.

[7] 徐亚军, 赵龙飞, 陈普, 等. 植物病原菌拮抗性野生艾蒿内生菌的分离、筛选和鉴定. 生态学报, 2013, 33(12): 3697-3705. Xu Y J, Zhao L F, Chen P, et al. Isolation, screening and characterization of phytopathogen antagonistic endophytes from wild Artemisia argyi. Acta Ecologica Sinica, 2013, 33 (12): 3697-3705.

[8] 王莉衡. 植物内生菌的研究进展. 化学与生物工程, 2011, 28(3): 5-7. Wang L H. Research advance on plant endophyte. Chemistry & Bioengineering, 2011, 28 (3): 5-7.

[9] Huss-Danell K. Actinorhizal symbioses and their N2 fixation. New Phytologist, 1997, 136(3): 375-405.

[10] 梁宗琦. 真菌次生代谢产物多样性及其潜在应用价值. 生物多样性, 1999, 7(2): 145-150. Liang Z Q. The diversity of fungal secondary metabolites and their potential applications. Chinese Biodiversity, 1999, 7(2): 145-150.

[11] 孙会强, 周赛男, 谭周进, 等. 一株烟草内生拮抗放线菌发酵条件优化. 应用与环境生物学报, 2011, 17(6): 914-917. Sun H Q, Zhou S N, Tan Z J, et al. Optimization of fermentation conditions of an endophytic actinomycetes from tobacco plant. Chin J Appl Environ Biol, 2011, 17 (6): 914-917.

[12] Conn V, Walker A, Franco C. Endophytic actinobacteria induce defense pathways in Arabidopsis thaliana. Molecular Plant-Microbe Interactions, 2008, 21(2): 208-218.

[13] Cao L, Qiu Z, You J, et al. Isolation and characterization of endophytic streptomycete antagonists of Fusarium wilt pathogen from surface-sterilized banana roots. FEMS Microbiol Lett, 2005, 247(2): 147-152.

[14] Kunoh H. Endophytic actinomycetes attractive biocontrol agents. Journal of General Plant Pathology, 2002, 68(3): 249-252.

[15] Shimizu M, Fujita N, Nakagawa Y, et al. Disease resistance of tissue-cultured seedlings of rhododendron after treatment with Streptomyces sp. R-5. Journal of General Plant Pathology, 2001, 67(4): 325-332.

[16] Shimizu M, Meguro A, Hasegawa H, et al. Disease resistance induced by nonantagonistic endophytic Streptomyces spp. on tissue-cultured seedlings of rhododendron. Journal of General Plant Pathology, 2006, 72(6): 351-354.

[17] Meguro A, Hasegawa S, Shimizu M, et al. Induction of disease resistance in tissue-cultured seedlings of mountain laurel after treatment with Streptomyces padanus AOK-30. Actinomycetologica, 2004, 18(2): 48-53.

[18] Hasegawa H, Meguro A, Toyoda K, et al. Drought tolerance of tissue-cultured seedlings of mountain laurel (Kalmia latifolia L.) induced by an Endophytic actinomycete II. acceleration of callose accumulation and lignification. Actinomycetologica, 2005, 19(1): 13-17.

[19] Meguro A, Toyoda K, Ogiyama H, et al. Genes expressed in tissue-cultured seedlings of mountain laurel (Kalmia latifolia L.) with colonizing Streptomyces padanus AOK-30. Journal of General Plant Pathology, 2012, 78(5): 303-310.

[20] Merzaeva O V, Shirokikh I G. The production of auxins by the endophytic bacteria of winter rye. Applied Biochemistry and Microbiology, 2010, 46(1): 44-50.

[21] El-Tarabily K A, Nassar A H, Hardy G E, et al. Plant growth promotion and biological control of Pythium aphanidermatum, a pathogen of cucumber, by Endophytic actinomycetes. J Appl Microbiol, 2009, 106(1): 13-26.

[22] Igarashi Y, Iida T, Furumai T. Pteridic acids a and b,novel plant growth promoters with auxi-like activity from Streptomyces hygroscopicus TP-A0451. J Antibiot (Tokyo), 2002, 55(8): 764-767.

[23] Shutsrirung A, Chromkaew Y, Pathom-Aree W, et al. Diversity of Endophytic actinomycetes in mandarin grown in northern thailand, their phytohormone production potential and plant growth promoting activity. Soil Science and Plant Nutrition, 2013, 59(3): 322-330.

[24] Goudjal Y, Toumatia O, Sabaou N, et al. Endophytic actinomycetes from spontaneous plants of Algerian Sahara: Indole-3-acetic acid production and tomato plants growth promoting activity. World J Microbiol Biotechnol, 2013, 29(10): 1821-1829.

[25] Nimnoi P, Pongsil P N, Lumyong S. Co-inoculation of soybean (Glycine max) with actinomycetes and Bradyrhizobium japonicum enhances plant growth, nitrogenase activity and plant nutrition. Journal of Plant Nutrition, 2014, 37(3): 432-446.

[26] Nimnoi P, Pongsil P N, Lumyong S. Endophytic actinomycetes isolated from Aquilaria crassna pierre ex Lec and screening of plant growth promoters production. World Journal of Microbiology and Biotechnology, 2009, 26(2): 193-203.

[27] Madhurama G, Sonam D, Urmil P G, et al. Diversity and biopotential of Endophytic actinomycetes from three medicinal plants in India. African Journal of Microbiology Research, 2014, 8(2): 184-191.

[28] El-Tarabily K A, Hardy G E, Sivasithamparam K. Performance of three Endophytic actinomycetes in relation to plant growth promotion and biological control of Pythium aphanidermatum, a pathogen of cucumber under commercial field production conditions in the united Arab emirates. European Journal of Plant Pathology, 2010, 128(4): 527-539.

[29] 刘志恒, 姜成林. 放线菌现代生物学与生物技术. 北京: 科学出版社, 2004. Liu Z H, Jiang C L. Modern Biology and Biotechnology of Actinomycetes. Beijing: Science Press, 2004.

[30] Callaham D, Deltredici P, Torrey J. Isolation and cultivation in vitro of the actinomycete causing root nodulation in Comptonia. Science, 1978, 199(4331): 899-902.

[31] Newcomb W, Peterson R l, Callaham D, et al. Structure and host-actinomycete interactions in developing root nodules of Comptonia peregrina. Canadian Journal of Botany, 1978, 56(5): 502-531.

[32] 杜大至, 王毅岩. 非豆科植物共生固氮的研究I.根瘤内生菌的观察方法. 微生物学报, 1983, 23(4): 347-350. Du D Z, Wang Y Y. Studies on symbiotic nitrogen fixation of non-leguminous plants I. a method for observation of the endophytic organism of the root nodules. Acta Microbiologica Sinica, 1983, 23 (4): 347-350.

[33] 徐丽华, 娄恺, 张华, 等. 微生物资源学. 北京: 科学出版社, 2010.9. Xu L H, Lou K, Zhang H, et al. Microbial Resources. Beijing: Science Press, 2010.9.

[34] 徐丽华, 李文均, 刘志恒, 等. 放线菌系统学——原理、方法及实践. 北京: 科学出版社, 2007. Xu L H, Li W J, Liu Z H, et al. Actinomycetes Systematics—Principle, Methods and Practice, Beijing: Science Press, 2007.

[35] Taechowisani T, Wanbanjob A, Tuntiwachwuttikul P, et al. Identification of Streptomyces sp TC022, an endophyte in Alpinia galanga, and the isolation of actinomycin. Annals of Microbiology, 2006, 56(2): 113-117.

[36] Yan L L, Han N N, Zhang Y Q, et al. Antimycin A18 produced by an endophytic Streptomyces albidoflavus isolated from a mangrove plant. J Antibiot (Tokyo), 2010, 63(5): 259-261.

[37] Castillo U, Harper J K, Strobel G A, et al. Kakadumycins, novel antibiotics from Streptomyces sp. NRRL 30566, an endophyte of Grevillea pteridifolia. FEMS Microbiology Letters, 2003, 224(2): 183-190.

[38] Inahashi Y, Iwatsuki M, Ishiyama A, et al. Spoxazomicins A-C, novel antitrypanosomal alkaloids produced by an endophytic actinomycete, Streptosporangium oxazolinicum K07-0460T. J Antibiot (Tokyo), 2011, 64(4): 303-307.

[39] Lu C, Shen Y. A new macroline antibiotic with antitumor activity produced by Streptomyces sp. CS, a commensal microbe of Maytenus hookeri. J Antibiot (Tokyo), 2003, 56(4): 415-418.

[40] Pullen C, Schmitz P, Meurer K, et al. New and bioactive compounds from streptomyces strains residing in the wood of Celastraceae. Planta, 2002, 216(1): 162-167.

[41] Niamsup H, Tang-um J. Chitinase production and antifungal potential of endophytic Streptomyces strain P4. Maejo International Journal of Science and Technology, 2012, 6(1): 95-104.

[42] Minamiyama H, Shimizu M, Kunoh H, et al. Multiplication of isolate R-5 of Streptomyces galbus on rhododendron leaves and its production of cell wall-degrading enzymes. Journal of General Plant Pathology, 2003, 69(1): 65-70.

[43] 蔡爱群, 田新莉, 周世宁. 水稻内生放线菌降解酶活性的分析. 韶关学院学报, 2007, 28(3): 107-109. Cai A Q, Tian X L, Zhou S N. Analysis of degration activities of Endophytic actinomycete from rice. Journal of Shaoguang University Natural Science, 2007, 28 (3): 107-109.

[44] Stamford T, Stamford N, Coelho L, et al. Production and characterization of a thermostable α-amylase from Nocardiopsis sp. Endophyte of yam bean. Bioresource Technology, 2001, 76(2): 137-141.

[45] El-Bondkly A M, El-Gendy M M. Keratinolytic activity from new recombinant fusantaya 2000, derived from endophytic micromonospora strains. Can J Microbiol, 2010, 56(9): 748-760.

[46] Stamford T, Stamford N, Coelho L, et al. Production and characterization of a thermostable glucoamylase from Streptosporangium sp. endophyte of maize leaves. Bioresource Technology, 2002, 83(2): 105-109.

[47] 杜慧镜, 苏静, 余利岩, 等. 药用植物内生放线菌的分离和生物学特性. 微生物学报, 2013, 53(1): 15-23. Du H J, Su J, Yu L Y, et al. Isolation and physiological characteristics of endophytic actinobacteria from medium plants. Acta Microbiologica Sinica, 2013, 53 (1): 15-23.

[48] Mergel C M, Robl D, de Oliveira Gregório Z M, et al. Bioprospecting lignocellulolytic enzymes from Endophytic actinomycetes aiming at second generation ethanol production. New Biotechnology, 2012, 29: S39.

[49] Lin C, Lu C, Shen Y. Three new 2-pyranone derivatives from mangrove Endophytic actinomycete strain Nocardiopsis sp. A00203. Records of Natural Products, 2010, 4(4): 176-179.

[50] Igarashi Y, Trujillo M E, Martinez-Molina E, et al. Antitumor anthraquinones from an endophytic actinomycete Micromonospora lupini sp. Nov. Bioorg Med Chem Lett, 2007, 17(13): 3702-3705.

[51] Yu Z, Zhao L X, Jiang C L, et al. Bafilomycins produced by an endophytic actinomycete Streptomyces sp. YIM56209. J Antibiot (Tokyo), 2011, 64(1): 159-162.

[52] Huang X L. Isolation and bioactivity of endophytic filamentous actinobacteria from tropical medicinal plants. African Journal of Biotechnology, 2012, 11(41): 9855-9864.

[53] Tuntiwachwuttikul P, Taechowisan T, Wanbanjob A, et al. Lansai A-D, secondary metabolites from Streptomyces sp. SUC1. Tetrahedron, 2008, 64(32): 7583-7586.

[54] Caruso M, Colombo A L, Crespi-Perellino N, et al. Studies on a strain of Kitasatospora sp. paclitaxel producer. Annals of Microbiology, 2000, 50(2): 89-102.

[55] Li J, Zhao G Z, Chen H H, et al. Antitumour and antimicrobial activities of endophytic streptomycetes from pharmaceutical plants in rainforest. Lett Appl Microbiol, 2008, 47(6): 574-580.

[56] 冯治翔, 栗敏, 刘洋, 等. 连翘和水茄内生放线菌的多样性、活性及生物合成基因的PCR筛查. 微生物学通报, 2011, 38(4): 539-546. Feng Z X, Li M, Liu Y, et al. Diversity, activity and PCR screening of biosynthesis genes of endophytic actinomycetes isolated from Forsythia suspensa. Microbiology China, 2011, 38 (4): 539-546.

[57] Huang S X, Yu Z, Robert F, et al. Cycloheximide and congeners as inhibitors of eukaryotic protein synthesis from endophytic actinomycetes Streptomyces sps. YIM56132 and YIM56141. J Antibiot (Tokyo), 2011, 64(1): 163-166.

[58] Nakashima T, Okuyama R, Kamiya Y, et al. Trehangelins a, b and c, novel photo-oxidative hemolysis inhibitors produced by an endophytic actinomycete, Polymorphospora rubra K07-0510. J Antibiot (Tokyo), 2013, 66(6): 311-317.

[59] Jasmine D J, Agastian P. In vitro antioxidant activity and in vivo alpha glucosidase activity of endophytic actinomycetes isolated from Catharanthus roseus (l.) G. Don. Journal of Pharmacy Research, 2013, 6(6): 674-678.

[60] Nimal Christhudas I V, Praveen Kumar P, Agastian P. In vitro alpha-glucosidase inhibition and antioxidative potential of an endophyte species (Streptomyces sp. Loyola UGC) isolated from Datura stramonium L. Curr Microbiol, 2013, 67(1): 69-76.

[1] 朱志坚, 连凯琪, 郑海学, 杨孝朴. 口蹄疫病毒入侵宿主细胞研究进展[J]. 中国生物工程杂志, 2015, 35(5): 103-108.
[2] 周兰, 吴晓龙, 付永卓, 赵相艳, 陈禹保, 谭钟扬. 有尾噬菌体目中微卫星和复合型微卫星的发生及分析[J]. 中国生物工程杂志, 2014, 34(11): 24-33.
[3] 梁小龙, 赵峰, 史荣久, 周纪东, 韩斯琴, 张颖. 原生质体融合构建高效产脂肽工程菌[J]. 中国生物工程杂志, 2014, 34(11): 76-84.
[4] 李多, 杨丽娟, 赵玉娇, 潘玥, 陈俊英, 付娟娟, 黄新伟, 邱丽娟, 孙强明. 研究报告重组Ⅱ型登革病毒NS1的表达及其免疫原性的研究[J]. 中国生物工程杂志, 2014, 34(9): 4-8.
[5] 夏启玉, 孙建波, 顾文亮, 卢娟, 卢雪花, 王宇光, 张欣. 香蕉内生克雷伯氏菌KKWB-5强启动子片段的分离及鉴定[J]. 中国生物工程杂志, 2011, 31(04): 37-43.
[6] 杨登峰 潘丽霞 关妮 米慧芝 左文朴 黄日波.
利用木糖-葡萄糖为原料产乙醇酵母的筛选、鉴定及发酵试验
[J]. 中国生物工程杂志, 2009, 29(10): 69-73.
[7] 汪小锋,杨江科,闫云君. 洋葱假单胞菌(Pseudomonas cepacia)PCL-3产脂肪酶发酵条件研究[J]. 中国生物工程杂志, 2008, 28(10): 72-78.