Please wait a minute...

中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志  2009, Vol. 29 Issue (10): 69-73    
研究报告     

利用木糖-葡萄糖为原料产乙醇酵母的筛选、鉴定及发酵试验
杨登峰,潘丽霞,关妮,米慧芝,左文朴,黄日波**
广西科学院国家非粮生物质能源工程技术研究中心 南宁 530003
Studies on Screening, Identification and Fermentation Characters of a Yeast Strain Fermentation Ethanol from Xylose-Glucose
YANG Deng-feng,PAN Li-xia,GUAN Ni,MI Hui-zhi,ZUO Wen-pu,HUANG Ri-bo
National Engineering Research Center for Nonfood Biorefinery,Guangxi Academy of Sciences, Nanning 530003, China
 全文: PDF(544 KB)   HTML
摘要:

建立筛选利用木糖为碳源产乙醇酵母模型,获得一株适合利用木质纤维素为原料产乙醇的酵母菌株。样品经麦芽汁培养基培养后,以木糖为唯一碳源的筛选培养基初筛,再以重铬酸钾显色法复筛。通过生理生化和26D1/D2区对筛选得到的菌株进行分析和鉴定,该菌初步鉴定为Pichia caribbica。经过筛选得到的菌株Y2-3以木糖(40g/L)为唯一碳源发酵时:生物量为23.5g/L,木糖利用率为94.7 %,乙醇终产量为4.57 g/L;以混合糖(葡萄糖40 g/L,木糖20 g/L)发酵时:生物量为28.6 g/L,木糖利用率为94.2 %,葡萄糖利用率为95.6%,乙醇终产量为20.6 g/L。Pichia caribbica是可以转化木糖及木糖-葡萄糖混合糖为乙醇的酵母菌株,为利用木质纤维素发酵乙醇的进一步研究奠定了基础。

关键词: 乙醇木质纤维素筛选鉴定    
Abstract:

A model for screening the yeast which can ferment xylose to produce the ethanol was constructed. An ethanol yeast was obtained using the lignocellulose as substrate production the ethanol. By malt extract medium preculturing, soil samples use the plate with xylose as sole carbon source as the primary screening, then finally screen by the potassium dichromate color-displaying method. A strain named Y2-3 was screened from the soil. Phenotypic analysis including morphology and physiology and biochemical characteristics and 26D1/D2 sequence analysis were carried out. Based on taxonomy results, the Y2-3 was identified as Pichia caribbica. The strain Y2-3 ferments using xylose as sole carbon source: biomass 23.5 g/L,xylose utilization rate 94.7 %,ethanol final yield 4.57 g/L;using mixture sugar:biomass 28.6 g/L,xylose utilization rate 94.2 %,glucose utilization rate 95.6%,ethanol final yield 20.6 g/L.Pichia caribbica is a yeast which can utilize xylose and mixture sugar as substrate.It established the foundation for further research fermentation of ethanol by yeast using lignocellulose.

Key words: Ethanol    Lignocellulose    Screening    Characterization
收稿日期: 2009-06-02 出版日期: 2009-10-25
ZTFLH:  Q939  
基金资助:

国家自然科学基金(20666002)、广西科技攻关项目(0537012)资助项目

通讯作者: 黄日波     E-mail: rbhuang@gxas.cn
服务  
把本文推荐给朋友
加入引用管理器
E-mail Alert
RSS
作者相关文章  
杨登峰
潘丽霞
关妮
米慧芝
左文朴
黄日波

引用本文:

杨登峰 潘丽霞 关妮 米慧芝 左文朴 黄日波.
利用木糖-葡萄糖为原料产乙醇酵母的筛选、鉴定及发酵试验[J]. 中国生物工程杂志, 2009, 29(10): 69-73.

YANG De-Feng, BO Li-Xia, GUAN Ni, MI Hui-Zhi, ZUO Wen-Piao, HUANG Ri-Bei. Studies on Screening, Identification and Fermentation Characters of a Yeast Strain Fermentation Ethanol from Xylose-Glucose. China Biotechnology, 2009, 29(10): 69-73.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/        https://manu60.magtech.com.cn/biotech/CN/Y2009/V29/I10/69

[1] Jeffries T W, Jin Y S. Metabolic engineering for improved fermentation of pentoses by yeasts. Appl Microbiol Biotechnol, 2004, 63(5): 495~509
[2] Martin Carlos, Galbe Mats, Wahlbom C Fredrik, et al. Ethanol production from enzymatic hydrolysates of sugarcane bagasse using recombinant xyloseutilising Saccharomyces cerevisiae. Enzyme and Microbial Technology, 2002, 31(3): 274~282
[3] 孔祥莉,刘波,赵宗保,等.斯达氏油脂酵母利用混合糖发酵产油脂.生物加工过程, 2007, 5(2): 36~41 Kong S L, Liu B, Zhao Z B, et al. Chinese Journal of Bioprocess Engineering, 2007, 5(2): 36~41
[4] 李阜棣,喻子牛,何绍江. 农业微生物学实验技术. 北京:中国农业出版社, 1996 Li F D, Yu Z N, He S J. Experimental Techniques of Agricultural Microbiology. Beijing: China Agriculture Press, 1996
[5] 刘慧. 现代食品微生物学实验技术. 北京:中国轻工业出版社,2006 Liu H. Experimental Techniques of Modern Food Microbiology. Beijing: China Light Industry Press, 2006
[6] 巴尼特.酵母菌的特征与鉴定手册.青岛:青岛海洋大学出版社,1991.17~31 Barnett J A. Yeasts:Characteristics and Identification. Qingdao: Qingdao Ocean University Press,1991.17~31
[7] Wyman C E, Dale B E, Elander R T, et al. Coordinated development of leading biomass pretreatment technologies. Bioresource Technology, 2005, 96(18): 1959~1966
[8] Yan Lin, Shuzo Tanaka. Ethanol fermentation from biomass resources: current state and prospects. Appl Microbiol Biotechnol, 2006, 69: 627~642
[9] Hideshi Yanase, Dai Sato, Keiko Yamamoto, et al. Genetic engineering of Zymobacter palmae for production of ethanol from xylose. Appled and Environmental Microbiology, 2007, 73(8): 2592~2599
[10] Cong T. Trinh, Pornkamol Unrean, Friedrich Srienc. Minimal Escherichia coil cell for the most efficient production of ethanol from hexoses and pentoses. Appled and Environmental Microbiology, 2008, 74(12): 3634~3643
[11] Shinuke Sakai, Yoshiki Tsuchida, Shohei Okino,et al. Effect of lignocellulose-derived inhibitors on growth of and ethanol production by growtharrested Corynebacterium glutamicum R. Appled and Environmental Microbiology, 2007, 73(7): 2349~2353
[12] JeanPaul Meijnen, Johannes H de Winde, Harald J Ruijssenaars. Engineering Pseudomonas putida S12 for efficient utilization of Dxylose and L-arabinose. Appled and Environmental Microbiology, 2008, 74(16): 5031~5037
[13] Sonderegger M, Sauer U. Evolutionary engineering of Saccharomyces cerevisiae for anaerobic growth on xylose. Appl Environ Microbiol, 2003, 69(4): 1990~1998
[1] 郭芳,张良,冯旭东,李春. 植物源UDP-糖基转移酶及其分子改造*[J]. 中国生物工程杂志, 2021, 41(9): 78-91.
[2] 钱昱,丁晓雨,刘志强,袁增强. 基因修饰人多能干细胞的高效单克隆建系方法[J]. 中国生物工程杂志, 2021, 41(8): 33-41.
[3] 翟君叶,成旭,孙泽敏,李春,吕波. 毛蕊花糖苷的生物合成研究进展[J]. 中国生物工程杂志, 2021, 41(5): 94-104.
[4] 高寅岭,张凤娇,赵贵众,张宏森,王风芹,宋安东. 衣康酸发酵研究进展[J]. 中国生物工程杂志, 2021, 41(5): 105-113.
[5] 张虎,刘镇洲,陈家敏,高保燕,张成武. 利用海洋硅藻生产生物活性物质研究进展*[J]. 中国生物工程杂志, 2021, 41(4): 81-90.
[6] 栗波,王泽建,梁剑光,刘爱军,李海东. 等离子体作用结合氧限制模型选育利福霉素SV高产菌株 *[J]. 中国生物工程杂志, 2021, 41(2/3): 38-44.
[7] 邓蕊,曾佳利,卢雪梅. 基于Musca domestica cecropin的抗肿瘤小分子衍生肽筛选及构效关系解析*[J]. 中国生物工程杂志, 2021, 41(11): 14-22.
[8] 范雁,杨淼,薛松. 基于光谱法-图像灰度法高通量筛选高效固定CO2的苯甲酸脱羧酶*[J]. 中国生物工程杂志, 2021, 41(11): 55-63.
[9] 察亚平, 朱牧孜, 李爽. 体内连续定向进化研究进展 *[J]. 中国生物工程杂志, 2021, 41(1): 42-51.
[10] 郭二鹏, 张建志, 司同. 羊毛硫肽的高通量工程改造方法新进展 *[J]. 中国生物工程杂志, 2021, 41(1): 30-41.
[11] 蔺士新,刘东晨,雷云,熊盛,谢秋玲. TNF-α纳米抗体的筛选、表达及特异性检测 *[J]. 中国生物工程杂志, 2020, 40(7): 15-21.
[12] 张野,王吉平,苏天明,何铁光,王瑾,曾向阳. 筛选微生物降解木质纤维素的研究进展[J]. 中国生物工程杂志, 2020, 40(6): 100-105.
[13] 董璐,张继福,张云,胡云峰. 环氧树脂固定化的Bacillus sp. DL-2胞外蛋白酶在拆分(±)-乙酸苏合香酯中的应用 *[J]. 中国生物工程杂志, 2020, 40(4): 49-58.
[14] 彭强强,刘启,徐名强,张元兴,蔡孟浩. 新型重组毕赤酵母产人胰岛素前体的表达工艺研究 *[J]. 中国生物工程杂志, 2019, 39(7): 48-55.
[15] 卢钟腾,呼高伟. 新型细胞穿膜肽的鉴定方法与其在抗肿瘤治疗中的应用[J]. 中国生物工程杂志, 2019, 39(12): 50-55.