| 研究报告 |
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| 可溶型三聚体血管生长抑制因子Kringle5的克隆,表达,纯化及活性研究 |
| 妙亮, 徐立华, 冀胥, 边六交 |
| 西北大学生命科学学院 西安 710069 |
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| Cloning, Expression, Purification and its Biological Activity Study of the Soluble Tripolymer Recombinant Angiogenesis Inhibitor Kringle 5 |
| MIAO Liang, XU Li-hua, JI Xu, BIAN Liu-jiao |
| College of Life Sciences, Northwest University, Xi’an 710069, China |
引用本文:
妙亮, 徐立华, 冀胥, 边六交. 可溶型三聚体血管生长抑制因子Kringle5的克隆,表达,纯化及活性研究[J]. 中国生物工程杂志, 2011, 31(03): 18-22.
MIAO Liang, XU Li-hua, JI Xu, BIAN Liu-jiao. Cloning, Expression, Purification and its Biological Activity Study of the Soluble Tripolymer Recombinant Angiogenesis Inhibitor Kringle 5. China Biotechnology, 2011, 31(03): 18-22.
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https://manu60.magtech.com.cn/biotech/CN/
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https://manu60.magtech.com.cn/biotech/CN/Y2011/V31/I03/18
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| [1] Cao Y, Tumor angiogenesis and therapy. Biomedicine & Pharmacotherapy, 2005, 59(2): 340-343.
[2] Ji W R,Barrientos L G,Llinas M,et al. Selective inhibition by Kringle 5 of human plasminogen on endothelial cell migration, an important process in angiogenesis. Biochem Biophys Res Commun, 1998, 247(2): 414-419.
[3] Ji W R,Castelkino F J,Chang Y, et al. Characterization of Kringle domains of angiostatin as antagonists of endothelial cell migration, an important process in angiogenesis. Faseb, 1998, 12(15): 1731-1738.
[4] Nguyen T M,Subramanian I V,Kelekar A,et al. Kringle 5 of human plasminogen,an angiogenesis inhibitor, induces both autophagy and apoptotic death in endothelial cells. Blood, 2007, 109(11): 4793-4802.
[5] 边六交, 党红艳, 杨晓燕. 原核表达融合型血管生成抑制剂Kringle 5发酵条件的优化和初步纯化. 高校化学工程学报, 2006, 20(5): 775-780. Bian L J,Dang H Y,Yang X Y, Journal of Chemical Engineering of Chinese Universities, 2006, 20(5): 775-780.
[6] 周涟, 罗进贤, 张添元. 血管生成抑制素基因工程大肠杆菌的高密度发酵研究. 微生物学报, 2003, 43(2): 228-235. Zhou L,Luo J X,Zhang T Y. Acta Microbiologica Sinica, 2003, 43(2): 228-235.
[7] 贾信贵, 边六交. 两步柱色谱法分离纯化重组肿瘤血管生长抑制因子Kringle 5. 色谱, 2007, 25(3): 344-347. Jia X G,Bian L J. Chinese Journal of Chromatography, 2007, 25(3): 344-347.
[8] 汪家政. 蛋白质技术手册. 北京: 科学出版社, 2000.23-24. Wang J Z. Protein Technical Manual. Beijing:Science Press, 2000.23-24.
[9] 陈浩, 陈于红, 张菁,等. 人纤溶酶原Kringle 5基因的克隆、表达及产物纯化和性质鉴定. 南京大学学报(自然科学版), 2001, 37(2): 218-222. Chen H,Chen Y H,Zhang J, et al.Journal of Nanjing University(Natural Sciences), 2001, 37(2): 218-222.
[10] 梁欣. 复杂蛋白质样品中目标组分柱层析分离纯化策略研究. 西安:西北大学,化工学院, 2009. Liang X. Isolation and purification strategy of target protein in binary-and ternary-protein mixture on column chromatography. Xi’an: Northwest University, College of Chemistry & Chemical Engineering, 2009.
[11] 吴鹏, 杨晓燕, 边六交. 一种从蛇毒中纯化神经生长因子的新工艺.色谱, 2004, 22(1): 1-4. Wu P,Yang X Y,Bian L J. Chinese Journal of Chromatography, 2004,22(1):1-4.
[12] 王亚妮,刘先宁. 重组人色素上皮细胞衍生因子融合蛋白的制备及其抑制新生血管活性的研究. 中国生物工程杂志, 2008, 28 (12): 12-17. Wang Y N, Liu X N. China Biotechnology, 2008, 28 (12): 12-17.
[13] Susan J, Janet M T. Principles of protein-protein interactions. Proc Natl Acad Sci, 1996, 93(1):13-20.
[14] 井明艳,孙建义. 蛋白质的折叠调控与包涵体的形成. 浙江大学学报(农业与生命科学版), 2004, 30 (6): 690-696. Jing M Y, Sun J Y. Journal of Zhejiang University(Agric. & Life Sci.), 2004, 30 (6): 690-696.
[15] Kazumasa S, MotoHisa O, Yuji G. Salt-dependent monomer-dimer equilibrium of bovine β-lactoglobulin at pH3. Protein Science, 2001, 10(11):2325-2335.
[16] Thongngam M, Mcclements D J. Influence of pH, ionic strength, and temperature on self-association and interactions of sodium dodecyl sulfate in the absence and presence of chitosan. Langmuir, 2005, 21(1):79-86.
[17] Welch W J. Role of quality control pathways in human diseases involving protein misfolding. Seminars in Cell and Developmental Biology, 2004, 15 (1): 31-38. |
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