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| Regulation Role of Light-controlled Expression Systems in Synthetic Biology |
LIU Ting-ting,ZHANG Ping,ZHANG Yue( ) |
| College of Bioengineering, Dalian Polytechnic University, Dalian 116034, China |
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Abstract Optogenetics is a new technology that combines genetics technology and light control technology to control biological processes with light. Based on the strategy of combining optogenetics and synthetic biology methods, researchers combined light as a sensing module with existing gene modules in organisms to form a new gene circuit, and designed a system that can dynamically regulate gene expression through light signals. As a new type of dynamic regulation switch with low cost, low toxicity and high flexibility, it has not only been applied in disease diagnosis, material synthesis and other fields, but also greatly promoted the progress of microbial metabolism and helped solve energy shortage problems. Since photoreceptors are indispensable components in optogenetics technology, this paper introduce several light-regulated systems used to control gene expression according to the photosensitive properties of different photoreceptors, and focus on their applications in regulating microbial system gene expression, metabolic pathways and drug delivery. Meanwhile, the potential problems and prospects of the application of photogenetics in synthetic biology were also discussed.
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Received: 11 October 2022
Published: 04 May 2023
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