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中国生物工程杂志

China Biotechnology
China Biotechnology
China Biotechnology  2020, Vol. 40 Issue (5): 94-104    DOI: 10.13523/j.cb.1912031
    
Recent Advances in Protein Expression System of Filamentous Fungi
HU Yi-bo1,2,**(),PI Chang-yu1,2,ZHANG Zhe1,2,XIANG Bo-yu1,2,XIA Li-qiu1,2
1 State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Science, Hunan Normal University, Changsha 410081, China
2 Hunan Provincial Key Laboratory of Microbial Molecular Biology, College of Life Science, Hunan Normal University, Changsha, 410081, China
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Abstract  

Filamentous fungi, which with excellent protein secretion and environmental adaptability, are widely used in protein expression fields. In recent decades, the expression efficiency of filamentous fungi including Aspergillus sp., Trichoderma sp. and Penicillium sp. has been continuously increased by means of mutagenesis,culture optimization and genetic modification. In order to promote the development of filamentous fungal protein expression system,filamentous fungi expression hosts, protein expression elements and their modification strategies were reviewed . At the same time, the disadvantage in the development of filamentous fungal expression system were discussed to provide references and insights for the study of novel filamentous fungi expression systems.

Key words： Filamentous fungi      Expression system      Protein expression      Host      Promoter     
Received: 18 December 2019      Published: 02 June 2020
ZTFLH:  Q816  
Corresponding Authors: Yi-bo HU     E-mail: huyibo@hunnu.edu.cn
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Yi-bo HU
Chang-yu PI
Zhe ZHANG
Bo-yu XIANG
Li-qiu XIA
Cite this article:

HU Yi-bo,PI Chang-yu,ZHANG Zhe,XIANG Bo-yu,XIA Li-qiu. Recent Advances in Protein Expression System of Filamentous Fungi. China Biotechnology, 2020, 40(5): 94-104.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.1912031     OR     https://manu60.magtech.com.cn/biotech/Y2020/V40/I5/94

Promotion type Promoter Source Reference Promoter Source Reference
Constitutive tpiA A. nidulans [47] pki T. reesei [48]
adhA A. nidulans [47] tef1 T. reesei [48]
gpdA A. nidulans [49] pgk1 T. reesei [48]
oliC A. nidulans [49] gpd T. reesei [50]
tef1 A. oryzae [51] cdna1 T. reesei [34]
mbfA A. niger [52] pdc T. reesei [53]
coxA A. niger [52] eno T. reesei [53]
srpB A. niger [52] ubiD P. oxalicum [23]
tvdA A. niger [52] pgmC P. oxalicum [23]
mdhA A. niger [52] aciA P. oxalicum [23]
manB A. niger [52] pcbAB P.chrysogenum [54]
bphA A. niger [55] pcbC P.chrysogenum [56]
rp2 T. reesei [57] SHO1 P.chrysogenum [56]
Conditional alcA A. nidulans [58] cbhI T. reesei [59]
oliC/acuD A. nidulans [49] cbhII T. reesei [60]
alcC A. nidulans [47] xyn2 T. reesei [61]
glaA A. niger [62] egl2 T. reesei [63]
gas A. niger [64] tcu1 T. reesei [65]
pacA A. niger [66] cbhI P. oxalicum [5]
exlA A. awamori [67] xylA P. chrysogenum [68]
thiA A.oryzae [69] ChiI M. thermophila [36]
Table 1 Promoters used for expression systems in filamentous fungi
Fig.1 Schematic diagram for improving target gene transcription with multiple promoters
Fig.2 Schematic diagram for improving target gene transcription with transactivator-promoter module
Fig.3 Schematic diagram of artificial synthetic promoter Expression of an TF (transcription factor)is achieved by a CP1 (core promoter), which provides low and highly constitutive expression. The TF, composed of AD (activate domain) and BD (binding domain), recognizes BS (binding sites) in the upstream part of a target gene artificial synthetic promoter, which containing variable number of BSs and different CP2. The drawing of this picture refers to the literature[78]
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