Research Progress on Techniques for Separation, Purification of Bacteriophages

doi:10.13523/j.cb.1912002

China Biotechnology

2020, Vol. 40

Issue (5): 78-83 DOI: 10.13523/j.cb.1912002

Research Progress on Techniques for Separation, Purification of Bacteriophages

QIN Xu-ying,YANG Hong-jiang(

)

Key Laboratory of Industrial Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China

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Abstract

Bacteriophage is widely used in many fields, so in the process of preparing phage, the different techniques or several techniques to obtain phage preparations with different purity to be used. The techniques commonly used to separate and purify phage are precipitation, filtration and centrifugation. In recent years, the application of chromatographic, field-flow fractionation techniques and electrophoresis techniques provides a new direction for obtaining phage preparations.

Key words： Bacteriophage Chromatography Field-flow fractionation Dielectrophoresis

Received: 01 December 2019 Published: 02 June 2020

ZTFLH:

Q815

Corresponding Authors: Hong-jiang YANG E-mail: hongjiangyang@tust.edu.cn

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Cite this article:

QIN Xu-ying,YANG Hong-jiang. Research Progress on Techniques for Separation, Purification of Bacteriophages. China Biotechnology, 2020, 40(5): 78-83.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.1912002 OR https://manu60.magtech.com.cn/biotech/Y2020/V40/I5/78

Fig.1 Principle of asymmetrical flow field-flow fractionation

Fig.2 Schematic representation of insulator-based dielectrophoresis channel


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