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| The Research Progress of Perfusion Mammalian Cell Culture |
Shuang SU1,Yong-jie JIN2,Rui-jing HUANG2,Jian LI2,**( ),Han-mei XU1,**() |
1 China Pharmaceutical University, Nanjing 211100, China
2 Tasly Biomedical Co., Ltd., Shanghai 201203, China |
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Abstract In the current environment of biopharmaceuticals, cost pressures, rapidly fluctuating market demands and growing competition among biosimilars, existing bio-manufacturing technologies are challenged, and so that biotechnology companies are increasingly driven to develop innovative solutions for highly flexible and cost-effective manufacturing. As one of the important processes in mammalian cell culture, perfusion culture has two advantages. Firstly, it can provide a stable environment favorable to cells by continuously removing by-products and adding nutrients, so that it can solve the problems of unstable protein amount or low expression level. Also, it can optimize capacity utilization and increase production efficiency by increasing volumetric productivity. This paper systematically reviewed the progress of perfusion culture for mammalian cell culture,and it provides reference for further development and application.
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Received: 03 September 2018
Published: 12 April 2019
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Corresponding Authors:
Jian LI,Han-mei XU
E-mail: lijian16@tasly.com;1037714870@qq.com
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