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| Effect of Salt-enhanced Culture on the Production of Neomycin by Streptomyces fradiae |
WANG Shan1,XUE Zheng-lian1,2,3,*( ),SUN Jun-feng1,WANG Fang1,ZHOU Jian1,LIU Yan1,2,3,WANG Zhou1,2,3 |
1 College of Biological and Chemical Engineering, Anhui Polytechnic University, Wuhu 241000, China
2 Anhui Engineering Technology Research Center of Microbial Fermentation, Wuhu 241000, China
3 Anhui Engineering Laboratory for Industrial Microbiology Molecular Breeding, Wuhu 241000, China |
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Abstract Objective: Streptomyces fradiae is the main production strain of the aminoglycoside antibiotic neomycin. Its neomycin B has strong antibacterial activity, anti-cancer and anti-HIV effects, and it is of great significance to increase the titer of neomycin B.Methods: We added different kinds and concentrations of inorganic salts to the medium to change the physical and chemical properties near the cell wall, osmotic pressure and C/N ratio of the medium under the condition of satisfying the salt ions required for the normal growth of microorganisms.Results: The degree of influence of different inorganic salts on the titer of neomycin B from high to low is (NH4)2SO4, NaCl, KCl and K2SO4 from high to low, and the titer of neomycin B reached the highest value of 15 864 U/mL when 60 mmol/L (NH4)2SO4 was added, the maximum yield of NaCl at 80 mmol/L concentration was 7 429.7 U/mL, which was 3.8 times and 0.82 times higher than that without adding inorganic salts. Then we added different concentrations of (NH4)2SO4 to the culture medium containing 80 mmol/L NaCl and sampled them regularly to detect the changes of amino nitrogen, reducing sugar, pH, TG, bacterial concentration and neomycin B titer, it was found that the consumption rate of amino nitrogen, reducing sugar and TG was increased at the concentration of 60 mmol/L (NH4)2SO4, the maximum specific growth rate of mycelium was 0.097 /h, the synthesis of neomycin B was accelerated and the yield was increased to 17 399 U/mL, at the same time, the mycelium was shortened and the sporulation was advanced.Conclusion: The salt-enhanced culture could be used as a morphological engineering method to improve the yield of neomycin B by changing the microscopic morphology of Streptomyces fradiae. It can also be used as a medium optimization strategy to increase the yield of neomycin B, which laid the foundation for further studies on increasing the yield of Streptomyces secondary metabolite.
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Received: 21 March 2021
Published: 03 August 2021
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Corresponding Authors:
Zheng-lian XUE
E-mail: xuezl@ahpu.edu.cn
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