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| Construction and Characterization of Electrochemical Biosensor based on Endotoxin Aptameer |
| YI Yu, WANG Min-jun, MEI Jian-feng, CHEN Jian-shu, ZHANG Yan-lu, YING Guo-qing |
| College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310014, China |
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Abstract A biosensor was constructed to detect the endotoxin.The amine-terminated aptamer was immobilized on the gold electrode surface covered in advance with a self-assembled monolayer (SAM) of 3-mercaptopropionic acid (MPA). The modification of the gold electrode was confirmed by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). It was found that MPA assembly time at 6 h in the gold electrode surface to form a stable self-assembled monolayer.The biosensor has a good linear relationship with concentration of endotoxin in the range of 0.001~0.1 EU/ml, it is possible to be applied for quality control in the actual biological sample.
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Received: 08 March 2017
Published: 25 August 2017
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