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| Development of An Electrochemical Aptasensor Basic on the ssDNA Aptamer of Ractopamine |
| ZHOU Ni1,2, CHEN Dan1,2, YAO Dong-sheng1,3, XIE Chun-fang1,3, LIU Da-ling1,2 |
1. Institute of Microbial Technology, Jinan University, Guangzhou 510632, China;
2. National Engineering Research Center of Genetic Medicine, Guangzhou 510632, China;
3. Department of Bio-engineering, Jinan University, Guangzhou 510632, China |
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Abstract Ractopamine was illegally used in stock farming widely. The ractopamine residues in animal tissues had a potential risk to human beings. Therefore, a sensitive and selective analytical method was developed for determination and monitoring of ractopamine. In this paper, a single-stranded DNA aptamer which could bind actopamine with high affinity was obtained by Isothermal Titration Calorimetry method. With this aptamer as a bio-recognizer, an electrochemical biosensor of detecting ractopamine was developed successfully. According to the differential pulse voltammetry analysis, a linear relationship was observed between the peak current value (ΔIp) and the log concentration of ractopamine ranging from 0.5 ng/ml to 1.0×102 ng/ml, and the correlation coefficient was 0.977 0. The detection limit was 0.1 ng/ml with a reaction time of 15 min. Ractopamine with a same concentration was detected 7 times and the RSD was 3.8%, it indicates the aptasensor have a good reproducibility. What' more, the aptasensor have a good specificity to RAC.
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Received: 22 October 2013
Published: 25 January 2014
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