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| The Experimental Study of Preventive Effects from hTM Gene for Neointima Formation and Stenosis in Rabbit Artery Injury-block Model |
| DA Yi1,HUANG Xiao-ling2,MENG Min1,LI Jing-dong1,SHI De2 |
1.The Affiliated Hospital of North Sichuan Medical College,Nanchong 637000,China
2.The First Affiliated Hospital of Chongqing Medical University,Chongqing 400014,China |
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Abstract Objective: To observe neointima formation in rabbit artery injury-block model which were transferred by pcDNA3.1/hTM plasmids. Methods: After transferred pcDNA3.1/hTM plasmid into rabbit artery by high-pressure injection, rabbit common iliac artery were cut and anastomosed again. The expression of hTM protein at vessels wall were examined with immunohistochemistry at 3d,7d,14d and 28d after operation. At the 14d and 28d after second operation, the inside diameter of anastomotic stoma and blood flow velocity were checked by color Doppler. The treated artery were sliced and stained by Verhoeff. Local neointima formation and the ratio stenosis of intervascular was calculated by computer. Result: The expression of hTM was at higher level in hTM gene treated group than vector treated group and control group at 3day, 7day, 14day, 28day after second operation. At 7day, the level was highest. At 14day and 28day, Color Doppler showed that the stoma bore were 1.93mm±0.34mm(14d),1.89mm±0.28mm(28d) in hTM gene treated group；1.59mm±0.43mm(14d),1.38mm±0.28mm(28d) in vector treated group；1.46mm0.25mm,1.44mm±0.32mm in control group. The ratio stoensis were 32%±23%(14d),37%±14%（28d） in hTM gene treated group；58%±21%(14d),63%±17% in vector treated group；58%±19%(14d),61%±23%(28d) in the control group，respectively. Conclusion: Human thrombmodulin gene could reduce the neointima formation and vascular stoensis in rabbit artery injury-block model.
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Received: 08 January 2010
Published: 12 June 2010
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Corresponding Authors:
dai yi
E-mail: chinadaiyi@yahoo.com.cn
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