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| Gene Cloning and Characters of Recombinant Methionine γ-Lyase from Trichomonas Vaginalis |
| XIA Li-liang1,HOU Wei2,WANG Bao-xue1,LIU Hua3,GAO Wei1,JIANG Qin1,LI Xin1,YU Yuan-hua1 |
1.College of Life Science, Changchun University of Science and Technology, Changchun 130022,China
2.NO.208 Hospital of Chinese People′s Liberation Army, Changchun 130021,China
3.Changchun Wei ErSai Bio-Pharmaceutical Co. Ltd., Changchun 130616,China |
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Abstract To explore the activity of recombinant Methionine γ-Lyase (MGL) , the gene of MGL was prepared by reverse PCR with RNA extracted from Trichomonas vaginalis. Expression vector pET-15b-mgl1 was constructed and sequenced. Characters of the recombinant MGL purified from transformed E. coli BL21 induced by IPTG were explored. The Km of the recombinant MGL for L-Methionine, D L-Homocysteine, L-Cystein as substrate were 0.318, 2.14, 1.62 mmol/L respectively and the specific activity were 20.17, 318.69, 56.96μmol/min/mg protein-respectively. The optimal pH for recombinant MGL was pH5.0~6.0 and the recombinant MGL was stable up to 50℃. The results indicate that recombinant MGL has bioactivity and good thermal stability suggesting potential efficacy in future clinical trials.
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Received: 11 January 2010
Published: 25 May 2010
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