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Cloning and Expression Analysis of JcAGG3, G-protein Gamma Subunitsthree Gene from Jatrophacurcas L. |
ZHANG Min, TIAN Yin-shuai, HU Xiao-le, XU Ying, CHEN Fang |
College of Life Sciences, Sichuan University, Chengdu 610064, China |
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Abstract An Arabidopsis thaliana AGG3 homologous gene was cloned from Jatrophacurcas L., which was named JcAGG3. JcAGG3 had an open reading frame of 834bp, encoding a protein of 277 amino acids. Its theoretical isoelectricand molecular weight point were 30.514kDa and 8.61 respectively. The protein was predictedlylocalised in plasma membrane.Through Biologysoftwareprediction analysis, a lot of promoter-binding elements related to the development of endosperm, hormonal regulation, light response and abiotic stresses were found in the cis-acting elements of the gene. The expression of JcAGG3 in different tissues and different development stages were examined using real-time polymerase chain reaction(RT-PCR). The results showed that JcAGG3 was expressed in root, stem, leaf and seed, with the highest expression in developing seed but least in stem. Young lealves had a significantly higher expression level than older leaves. After 6h dark treatment, the level of JcAGG3 expression was significantly reduced. However, under abscisicacid(ABA) and drought treatment, the level of JcAGG3 expression were increased.
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Received: 03 December 2015
Published: 04 January 2016
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