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中国生物工程杂志

China Biotechnology
China Biotechnology  2016, Vol. 36 Issue (5): 46-52    DOI: 10.13523/j.cb.20160507
    
Cloning and Expression Analysis of JcAGG3, G-protein Gamma Subunitsthree Gene from Jatrophacurcas L.
ZHANG Min, TIAN Yin-shuai, HU Xiao-le, XU Ying, CHEN Fang
College of Life Sciences, Sichuan University, Chengdu 610064, China
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Abstract  

An Arabidopsis thaliana AGG3 homologous gene was cloned from Jatrophacurcas L., which was named JcAGG3. JcAGG3 had an open reading frame of 834bp, encoding a protein of 277 amino acids. Its theoretical isoelectricand molecular weight point were 30.514kDa and 8.61 respectively. The protein was predictedlylocalised in plasma membrane.Through Biologysoftwareprediction analysis, a lot of promoter-binding elements related to the development of endosperm, hormonal regulation, light response and abiotic stresses were found in the cis-acting elements of the gene. The expression of JcAGG3 in different tissues and different development stages were examined using real-time polymerase chain reaction(RT-PCR). The results showed that JcAGG3 was expressed in root, stem, leaf and seed, with the highest expression in developing seed but least in stem. Young lealves had a significantly higher expression level than older leaves. After 6h dark treatment, the level of JcAGG3 expression was significantly reduced. However, under abscisicacid(ABA) and drought treatment, the level of JcAGG3 expression were increased.



Key wordsAGG3      Seed size      Gene cloning      Expression analysis      Jatrophacurcas L.     
Received: 03 December 2015      Published: 04 January 2016
ZTFLH:  Q789  
Cite this article:

ZHANG Min, TIAN Yin-shuai, HU Xiao-le, XU Ying, CHEN Fang. Cloning and Expression Analysis of JcAGG3, G-protein Gamma Subunitsthree Gene from Jatrophacurcas L.. China Biotechnology, 2016, 36(5): 46-52.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20160507     OR     https://manu60.magtech.com.cn/biotech/Y2016/V36/I5/46

[1] Foidl N, Foidl G, Sanchez M, et al. Jatrophacurcas L. as a source for the production of biofuel in Nicaragua. Bioresource Technology, 1996, 58(1): 77-82.
[2] Openshaw K. A review of Jatropha curcas: an oil plant of unfulfilled promise. Biomass and Bioenergy, 2000, 19(1): 1-15.
[3] Sujatha M, Reddy T P, Mahasi M J. Role of biotechnological interventions in the improvement of castor (Ricinuscommunis L.) and Jatrophacurcas L. Biotechnology Advances, 2008, 26(5): 424-435.
[4] Achten W M, Nielsen L R, Aerts R, et al. Towards domestication of Jatropha curcas. Biofuels, 2010, 1(1): 91-107.
[5] Singh K, Singh B, Verma S K, et al. Jatrophacurcas: a ten year story from hope to despair. Renewable and Sustainable Energy Reviews, 2014, 35: 356-360.
[6] Gilman A G. G proteins: transducers of receptor-generated signals. Annual Review of Biochemistry, 1987, 56(1): 615-649.
[7] Rodbell M. Nobel Lecture. Signal transduction: evolution of an idea. Bioscience reports, 1995, 15(3): 117-133.
[8] Urano D, Chen J G, Botella J R, et al. Heterotrimeric G protein signalling in the plant kingdom. Open Biology, 2013, 3(3): 120186.
[9] Ashikari M, Wu J, Yano M, et al. Rice gibberellin-insensitive dwarf mutant gene Dwarf 1 encodes the α-subunit of GTP-binding protein. Proceedings of the National Academy of Sciences, 1999, 96(18): 10284-10289.
[10] Fujisawa Y, Kato T, Ohki S, et al. Suppression of the heterotrimeric G protein causes abnormal morphology, including dwarfism, in rice. Proceedings of the National Academy of Sciences, 1999, 96(13): 7575-7580.
[11] Ullah H, Chen J G, Temple B, et al. The β-subunit of the Arabidopsis G protein negatively regulates auxin-induced cell division and affects multiple developmental processes. The Plant Cell, 2003, 15(2): 393-409.
[12] Li S, Liu Y, Zheng L, et al. The plant-specific G protein γ subunit AGG3 influences organ size and shape in Arabidopsis thaliana. New Phytologist, 2012, 194(3): 690-703.
[13] Roy Choudhury S, Riesselman A J, Pandey S. Constitutive or seed-specific overexpression of Arabidopsis G-protein γ subunit 3(AGG3) results in increased seed and oil production and improved stress tolerance in Camelina sativa. Plant Biotechnology Journal, 2014, 12(1): 49-59.
[14] Chakravorty D, Trusov Y, Zhang W, et al. An atypical heterotrimeric G-protein γ-subunit is involved in guard cell K+-channel regulation and morphological development in Arabidopsis thaliana. The Plant Journal, 2011, 67(5): 840-851.
[15] Mohapatra S, Panda P K. Genetic variability on growth, phenological and seed characteristics of Jatrophacurcas L. NotulaeScientiaBiologicae, 2010, 2(2): 127-132.
[16] Shabanimofrad M, Rafii M Y, Wahab P E M, et al. Phenotypic, genotypic and genetic divergence found in 48 newly collected Malaysian accessions of Jatrophacurcas L. Industrial Crops and Products, 2013, 42(1): 543-551.
[17] Jiang H, Wu P, Zhang S, et al. Global analysis of gene expression profiles in developing physic nut (Jatrophacurcas L.) seeds. PLoS One, 2012, 7(5): e36522.
[18] Scott R J, Spielman M, Bailey J, et al. Parent-of-origin effects on seed development in Arabidopsis thaliana. Development, 1998, 125(17): 3329-3341.
[19] Luo M, Dennis E S, Berger F, et al. MINISEED3(MINI3), a WRKY family gene, and HAIKU2(IKU2), a leucine-rich repeat (LRR) KINASE gene, are regulators of seed size in Arabidopsis. Proceedings of the National Academy of Sciences of the United States of America, 2005, 102(48): 17531-17536.
[20] Zhou Y, Zhang X, Kang X, et al. SHORT HYPOCOTYL UNDER BLUE1 associates with MINISEED3 and HAIKU2 promoters in vivo to regulate Arabidopsis seed development. The Plant Cell, 2009, 21(1): 106-117.
[21] Johnson C S, Kolevski B, Smyth D R. TRANSPARENT TESTA GLABRA2, a trichome and seed coat development gene of Arabidopsis, encodes a WRKY transcription factor. The Plant Cell, 2002, 14(6): 1359-1375.
[22] Jofuku K D, Omidyar P K, Gee Z, et al. Control of seed mass and seed yield by the floral homeotic gene APETALA2. Proceedings of the National Academy of Sciences of the United States of America, 2005, 102(8): 3117-3122.
[23] Ohto M, Floyd S K, Fischer R L, et al. Effects of APETALA2 on embryo, endosperm, and seed coat development determine seed size in Arabidopsis. Sexual plant reproduction, 2009, 22(4): 277-289.
[24] Schruff M C, Spielman M, Tiwari S, et al. The AUXIN RESPONSE FACTOR 2 gene of Arabidopsis links auxinsignalling, cell division, and the size of seeds and other organs. Development, 2006, 133(2): 251-261.
[25] Ye J, Liu P, Zhu C, et al. Identification of candidate genes JcARF19 and JcIAA9 associated with seed size traits in Jatropha. Functional & Integrative Genomics, 2014, 14(4): 757-766.

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