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| Cloning and Expression Analysis of V-type Proton ATPase Subunit a3 Gene in Sunflower (Helianthus annuus L.) |
| ZHANG Yan-fang1, SUN Rui-fen2, GUO Shu-chun2, HOU Jian-hua1 |
1. Agronomy College, Inner Mongolia Agricultural University, Huhhot 010019, China;
2. Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Huhhot 010031, China |
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Abstract The full length cDNA sequence of V-type proton ATPase subunit a3 gene was cloned from sunflower P50 by RACE. The sequencing result showed that full length cDNA(2 873bp) contain the 2 469bp open reading frame (ORF), encoding a 822 amino acids, 109bp 5'-untranslated region and 295bp 3'-untranslated region. It was a hydrophobic transmembrane protein and the predicted protein's molecular weight was 204.55kDa, isoelectric point was 6.29, GenBank accession number was KU315054. The protein had highly similar conserved region between sunflower and other 10 kinds of plants. The genetic relationship of sunflower was closest to Cynara cardunculus var. scolymus. RT-qPCR showed that the gene was up-regulated under different concentrations of NaCl, ABA and PEG in sunflower with different expression patterns in different conditions and organs. The research suggested that the V-type proton ATPase subunit a3 gene of sunflower responded to abiotic stresses, which laid the foundation for strengthening its utilization.
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Received: 08 December 2016
Published: 25 May 2017
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