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中国生物工程杂志

China Biotechnology
China Biotechnology  2015, Vol. 35 Issue (8): 44-50    DOI: 10.13523/j.cb.20150807
    
Cloning and Expression Analysis One Zinc Finger Protein Gene FtLOL1 in Fagopyrum tataricum: Effect of Abiotic Stress
GAO Fei, ZHOU Jing, LIU Xiao-tong, LI Cheng-lei, YAO Hui-peng, ZHAO Hai-xia, WU Qi
College of Life Science, Sichuan Agricultural University, Ya'an 625014, China
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Abstract  

According to transcriptome data of Fagopyrum tataricum at flowering, using PCR and RT-PCR techniques, the DNA and cDNA sequences of FtLOL1 (GenBank accession number: KP260662) gene were amplified from Fagopyrum tataricum. The obtained sequences were analyzed by bioinformatics software, and the expression FtLOL1 gene were analysed by qPCR under UV-B, SA and 4℃ cold stress. The results showed that the DNA sequence of FtLOL1 gene was 1 720bp, of which consisted 5 exons and 4 introns, in line with the principle of GT-AG splicing, and the cDNA of FtLOL1 contained a 444bp ORF, encoding 147 amino acids, with the typical features of LSD1 family. The 2mmol/L SA, UV-B radiation and 4℃ cold stress could lead to a significant discrease in the expression of the FtLOL1 gene. The results expected to lay a foundation for the stress resistance study in Fagopyrum tataricum.



Key wordsFagopyrum tataricum      Zinc finger protein LSD1      Gene cloning      Abiotic stress      Gene expression     
Received: 11 March 2015      Published: 25 August 2015
ZTFLH:  Q789  
Cite this article:

GAO Fei, ZHOU Jing, LIU Xiao-tong, LI Cheng-lei, YAO Hui-peng, ZHAO Hai-xia, WU Qi . Cloning and Expression Analysis One Zinc Finger Protein Gene FtLOL1 in Fagopyrum tataricum: Effect of Abiotic Stress. China Biotechnology, 2015, 35(8): 44-50.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20150807     OR     https://manu60.magtech.com.cn/biotech/Y2015/V35/I8/44


[1] 宋冰, 洪洋, 王丕武, 等. 植物 C2H2 型锌指蛋白的研究进展. 基因组学与应用微生物学, 2010, 29(5): 1133-1141. Song B, Hong Y, Wang P W, et al. Advances on plant C2H2-type zinc finger protein. Genome ApplBiol, 2010, 29(5): 1133-1141.

[2] 向建华, 李灵之, 陈信波. 植物非生物逆境相关锌指蛋白基因的研究进展. 核农学报, 2012,26(4): 666-672. Xiang J H, Li L Z, Chen X B. Progress in the study of abiotic stress-related zinc finger protein genes in plants. J Nuclear Agri Sci, 2012, 26(4): 666-672.

[3] Brown R S. Zinc finger proteins: getting a grip on RNA. Curr Opin Struct Biol, 2005, 15(1): 94-98.

[4] Dietrich R A, Richberg M H, Schmidt R, et al. A novel zinc finger protein is encoded by the Arabidopsis LSD1 gene and functions as a negative regulator of plant cell death. Cell, 1997, 88(5): 685-694.

[5] Torres M A, Jones J D, Dang J L, et al. Pathogen-induced, NADPH oxidase-derived reactive oxygen intermediates suppress spread of cell death in Arabidopsis thaliana. Nature Genetics, 2005, 37(10): 1130-1134.

[6] 王丽娟, 田颖川, 何朝族, 等. 新基因水稻OsLSD1的克隆及拟南芥和水稻类 LSD1基因家族的生物信息学分析. 生物化学与生物物理进展, 2005, 32(3): 268-274. Wang L J, Tian Y C, He C Z, et al. Cloning of a novel rice gene OsLSD1 and bioinformatic analysis of LSD1 -like gene family from Arabidopsis and Rice. Progress in Biochemistry and Biophysics, 2005, 32(3): 268-274.

[7] 黄新杰, 郭军, 屈志朋, 等. 小麦TaLSD1锌指蛋白基因的电子克隆及序列分析.西北植物学报, 2007, 27(11): 2147-2152. Huang X J, Guo J, Qu Z P, et al. In silico cloning and sequence analysis of a TaLSD1 zinc finger protein gene from wheat infected by stripe rust fungus. Acta Botanica Boreali-Occidentalia Sinica, 2007, 27(11): 2147-2152.

[8] Sheng-Hsiung Yeh, Choun-Sea Lin, Fu-Hui Wu, et al. Analysis of the expression of BohLOL1, which encodes an LSD1-like zinc finger protein in Bambusa oldhamii. Planta, 2011, 234:1179-1189.

[9] Calabrò M L, Tommasini S, Donato P, et al. The rutin/bcyclodextrin interactions infully aqueous solution:Spectroscopicstudies andbiological assays. J Pharm Biomed Anal, 2005, 36(5):1019-1027.

[10] Zwirtes O I, Fernandes S C, Vieira I C. Development of abiosensor basedongilo peroxidase immobilized on chitosan chemically crosslinked with epichlorohydrin for determination of rutin. J Pharm Biomed Anal, 2006, 41(2):366-372.

[11] Kitabayashi H, Ujihara A, Hirose T, et al. On the genotypic differences for rutin content in tatary buckwheat, Fagopyrum tataricum Gaertn. Breeding Sci, 1995, 45(2): 189-194.

[12] Suzuki T, Honda Y, Mukasa Y J. Effects of UV-B radiation, cold and desiccation stress on rutin concentration and rutin glucosidase activity in tartary buckwheat (Fagopyrum tataricum) leaves. Plant Science, 2005, 168: 1303-1307.

[13] 董新纯, 赵世杰, 郭珊珊, 等. 增强UV-B条件下类黄酮与苦荞逆境伤害和抗氧化酶的关系. 山东农业大学学报, 2006, 37(2): 157-162. Dong X C, Zhao S J, Guo S S, et al. Role of flavonoids on stress injury and antioxydative enzymes in Fagopyrum tataricun seedings under enhanced UV-B radiation. Journal of Shangdong Agricultural University, 2006, 37(2): 157-162.

[14] 李成磊, 冯争艳, 白悦辰, 等. 金荞麦苯丙氨酸解氨酶基因FdPAL的克隆及原核表达.中国中药杂志, 2011,36(23): 3238-3243. Li C L, Feng Z Y, Bai Y C, et al. Molecular cloning and prokaryotic expression of phenylalanine ammonialyase gene FDPAL from Fagopyrum dibotrys. China Journal of Chinese Materia Medica, 2011, 36(23): 3238-3243.

[15] Bai Y C, Li C L, Zhang J W, et al. Characterization of two tartary buckwheat R2R3-MYB transcription factors and their regulation of proanthocyandin biosynthesis. Physiologia Plantarum, 2014, 152(3): 431-440.

[16] Thomas D S, Kenneth J L. Analyzing real-time PCR data by the comparative CT method. Nature Protocols, 2008, 3 (6): 1101-1108.

[17] Epple P, Mack A A, morrisV R, et al. Antagonistic control of oxidative stress-induced cell death in Arabidopsis by two related, plant-specific zinc finger proteins. Proc Natl Acad Sci U S A, 2003, 100(11):6831-6836.

[18] Liu Q P,Xue Q Z.Molecular phylogeny, evolution, and functional divergence of the LSD1-like gene family: inference from the rice genome. Journal of Molecular Evolution,2007,64(3);354-363.

[19] Tatusov R L, Koonin E V, Lipman D J. A genomic perpective on protein families. Science, 1997, 278(5338):631-637.

[20] Danie H A, Christine R A, Ben F H, et al. Runaway cell death, but not basal disease resistance, in lsd1 is SA- andNIM1/NPR1-dependent. The Plant Journal, 2002, 29(3): 381-391.

[21] Kaminaka H, Nakc C, Epple P, et al. bZIP10-LSD1 antagonism modulates basal defense and cell death in Arabidopsis following infection. EMBO J, 2006, 25(18):4400-4411.

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