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| The Transcriptional Regulation of Ca2+ Channel Mediated by Myocardin in H9C2 Cell |
| DAI Yu huan1,2, XU Yao1, LUO Ying1, DAI Yang1, SHI Wei lin1, XU Yao1 |
1. Biomedical Institute, Wuhan University of Science and Technology, Wuhan 430065, China;
2. Wuhan Puren Hospital, Wuhan 430080, China |
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Abstract Objective:To investigate the effect of transcription factor Myocardin on the transcriptional regulation of L calcium channel Cav1.2 and its molecular mechanism in the process of maintaining normal structure and function of the heart. Methods:Cardiomyocyte membrane Ca2+ current is detected by electrophysiological patch clamp technique. Real-time PCR is used to detect the level of the LTCC mRNA,The protein expression level of LTCC is detected by Western blotting, Using luciferase assay to detect the promoter activity of Cav1.2 and the binding site of Myocardin on Cav1.2 gene promoter. Results:The activation of LTCC Cav1.2 gene mediated by Myocardin up-regulate the cardiomyocyte membrane Ca2+ current. Myocardin activates LTCC Cav1.2 gene transcription and expression depending on its CarGbox on the promoter. Conclusions:Myocardin activates LTCC Cav1.2 gene transcription and expression by binding on the CarGbox of its promoter, which promote the transportion of calcium ion channel protein from nucleus to membrane, enhance the flow of Ca2+, and up-regulate the cardiomyocyte membrane Ca2+ current.
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Received: 09 May 2016
Published: 25 November 2016
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