Advances in Cell Surface Display Technology in Environmental Remediation

doi:10.13523/j.cb.2205046

China Biotechnology

2022, Vol. 42

Issue (9): 105-115 DOI: 10.13523/j.cb.2205046

Advances in Cell Surface Display Technology in Environmental Remediation

HUANG Ming-zhu^1,²,YAO Kun²,SONG Zhuo-lin²,ZHANG Hao²,LIU Bin²,CHEN Xue-lan^1,^2,^**(

)

1. National R&D Center for Freshwater Fish Processing, Nanchang 330022, China
2. College of Life Sciences, Jiangxi Normal University, Nanchang 330022, China

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Abstract

Industrialization has led to various toxic compounds emission, which causes environmental contamination. Aside from natural geological weathering, traditional solutions include chemical conversion, physical absorption, electrochemical methods and ions exchange, but their applications are limited due to some disadvantages, such as secondary pollution, high energy requirement, high investment cost, low regeneration efficiency and inefficiency in low-concentration wastewater treatment. Cell surface engineering is an innovative, cost-effective biotechnology of microorganism for modification of cell surface function through joining external functional peptides with surface anchoring proteins. In contrast to conventional intracellular and secretion expression systems, proteins displayed on the surface of microorganism may exhibit enhanced stability against changes in organic solvents, proteases, temperature and pH. Surface-engineered cells prepared by cultivation are ready to be used as microparticles covered with proteins/peptides, avoiding troublesome concentration procedures and protein purification. Furthermore, cell-surface display engineering is suitable for high throughput screening from the mutant library for more capable proteins/peptides at the single-cell level. Currently, this technology is widely used in the control of environmental pollution. This review is focused on recent strategies of using cell-surface display technology in environmental bioremediation, summarizing its applications, recent progress and future prospects.

Key words： Bioremediation Biosorption Cell surface display Whole cell biocatalyst Microorganism

Received: 23 May 2022 Published: 10 October 2022

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Corresponding Authors: Xue-lan CHEN E-mail: xuelanchen162@163.com

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	Ming-zhu HUANG
	Kun YAO
	Zhuo-lin SONG
	Hao ZHANG
	Bin LIU
	Xue-lan CHEN

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HUANG Ming-zhu,YAO Kun,SONG Zhuo-lin,ZHANG Hao,LIU Bin,CHEN Xue-lan. Advances in Cell Surface Display Technology in Environmental Remediation. China Biotechnology, 2022, 42(9): 105-115.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.2205046 OR https://manu60.magtech.com.cn/biotech/Y2022/V42/I9/105

Fig.1 Cell surface display system^[5]

Fig.2 The construction route of the synthetic bacterium-MNP co-assemblies for adsorption of heavy metals^[21] SynHMB: The synthetic heavy-metal-binding protein; PEI: Polyethyleimine; DTPA: Diethylenetriaminepentaacetic acid; RBS: Ribosome binding sequence; pT7: T7 promoter; impX-impH: The gene cluster encoding T6SS; tT7: T7 terminator; Omp: OmpA secretory sequence; CTR: C-terminally transmembrane regions of OmpA; KanR: Kanamycin resistant gene; GFP: Green fluorescence protein-encoding gene; MNP: Magnetic nanoparticle; TEOS: Tetraethyl orthosilicate; MNP@SiO₂-PEI-DTPA: Silica-coated magnetic nanoparticle grafted with PEI and DTPA

Fig.3 Genetic circuit for bioremediation and biocontainment system ^[24]

Fig.4 Hg²⁺ of bioremediation and biocontainment system ^[25] Sensor 1: Hg²⁺ responsive module; Sensor 2: IPTG responsive module; MBP: Mercury binding protein; Toxin: Suicide protein; P_c: Constitutive promoter; P_MerT: Mercury inducible promoter; P_Tac: IPTG inducible synthetic promoter; P_LtetO: A well-known artificial promoter with extremely low basal leakage and negatively regulated by TetR

Table 1 Cell surface display system for recent applications in environmental remediation


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