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| Identification and Biological Characteristics of an Active Endophytic Fungus EZG0807 |
| YAN Ju-fen, QI Ning-bo, WANG Su-ping, GAI Li-li, YANG Shu-lin |
| The School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China |
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Abstract An active endophytic fungus EZG0807 isolated from the Chinese traditional medical plant Curcuma wenyujin was identified as Gibberella moniliformis through the molecular biology technique combined with morphology. It is a facultative endophytic fungus, which can not only induce the disease in plants and insects, but also stimulate the growth of host plant. In the other hand, the researches about the biological characters and fermentation conditions of this active fungus were carried out. It could use a variety of media except galactose and (NH4)2SO4and grew in the range of 15～35℃ and pH 4.0～10.0. The optimum growth temperature and pH were 30℃ and 7, respectively. Based on the fungal characteristics and previous one-factor experiment, the optimal fermentation condition was 28.72℃ for the temperature, 6.76 for initial pH and 10.02 d performed by the Response Surface Methodology. Under this optimal condition, the inhibition zone of fungal fermentation broth against Proteus vulgaris reached 25.6 mm, which was improved by 24.9%, making a foundation for further study on its metabolites.
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Received: 15 July 2013
Published: 25 October 2013
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