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| Experiment Study of Non-mitogenetic Human Acidic Fibroblast Growth Factor on Improving Scald Wound Healing in Mice |
| SHEN Juan1,2, JIN Xiao-bao2, Ding Jing2, ZHU Jia-yong 2 |
1. School of Life Science & Biopharmacology, Guangdong Pharmaceutical University Guangzhou 510006, China;
2. Guangdong Key Laboratory of Pharmaceutical Bioactive Substances, Guangzhou 510006, China |
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Abstract Objective To investigate the influence of non-mitogenetic human acidic fibroblast growth factor (nmhaFGF) on scald wound healing in mice. Method The recombinant protein nmhaFGF was expressed in Escherichia coli and purified. Mitogenic activity of nmhaFGF was analysed by Methylthiazoletetrazolium (MTT) assay. Deep II degree scald on the back in the mice was used as model. The mice were divided into nmhaFGF group( 20 μg nmhaFGF/cm2,PBS diluted) and control group( PBS). The time of wound healing was recorded, The wound area was measured at 1,3,7,9,14 d after injuring. Pathological examination with HE staining and immunohistochemistry for proliferating cell nuclear antigen ( PCNA) and collagen was observed at the 7th and 14th day after injuring. Result NmhaFGF of 95.10% was obtained. Compared to haFGF, the nmhaFGF showed the weaker activity. nmhaFGF can promote reepithelization in burn wounds. Compared with the control group, nmhaFGF group showed a shorter time (from 17.2 d to15.8 d) of wound healing (P<0.01). The histomorphometric results indicate that granulation tissue grew better and the expression level of PCNA is higher in nmhaFGF group at the 7th day. Epidermis grew faster and arrangement of collagen was more clean in nmhaFGF group at 21th day. Conclusion Topical application of nmhaFGF can promote reepithelization of deep II degree scald wounds and shorten the healing time of burns in mice.
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Received: 28 October 2012
Published: 25 March 2013
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[1] Xie L Y, Zhang M L, Dong B J, et al. Improved refractory wound healing with administration of acidic fibroblast growth factor in diabetic rats. Diabetes Research and Clinical Practice, 2011 93(3): 396-403.
[2] Smith G, Ng M T, Shepherd L, et al. Individuality in FGF1 expression significantly influences platinum resistance and progression-free survival in ovarian cancer. Br J Cancer, 2012, 107(8):1327-1336.
[3] Lozano R M, Pineda-Lucena A, Gonzalez C, et al. 1H NMR structural characterization of a nonmitogenic,vasodilatory, ischemia-protector and neuromodulatory acidic fibroblast growth factor. Biochemistry, 2000, 39 (17):4982-4993.
[4] Ching Y H, Sutton T L, Pierpont Y N, et al. The Use of Growth Factors and Other Humoral Agents to Accelerate and Enhance Burn Wound Healing. Eplasty, 2011, 11-41.
[5] Xu H, Li X K, Zheng Q, et al. Protective effects of mutant of acidic fibroblast growth factor against cerebral ischaemia-reperfusion injury in rats. Injury, 2009, 40(9): 963–967.
[6] Chen W W, Yu M, Peng Y W, et al. Non-mitogenic human acidic fibroblast growth factor reduces retinal degeneration induced by sodium iodate. Ocul Pharmacol Ther, 2009, 25(4): 315-319.
[7] Lin S Q, Xu H W, Xiao J, et al. Combined Use of Acid Fibroblast Growth Factor, Granulocyte Colony-stimulating Factor and Zinc Sulphate Accelerates Diabeltic Ulcer Healing. Journal of Health Science, 2009, 55(6):910-922.
[8] Wu X P, Su Z J, Li X K, et al. High-level expression and puriWcation of a nonmitogenic form of human acidic fibroblast growth factor in Escherichia coli. Protein Expr. Purif., 2005, 42(1): 7-11.
[9] Xie L Y, Zhang M L, Dong B J, et al. Improved refractory wound healing with administration of acidic fibroblast growth factor in diabetic rats. Diabetes Research and Clinical Practice, 2011,93(3): 396-403.
[10] 文辉才,巫国辉,陈雯等.局部应用瘦素对大鼠烫伤创面愈合的实验研究.南方医科大学学报,2012,32(5):703-706. Wen H C, Wu G H, Chen W, et al. Topical application of leptin promotes burn wound healing in rats. Journal of Southern Medical University, 2012,32(5):703-706. |
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