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| A Modified CTAB Method for Isolating Genome DNA from Fungus with Abundant Polysaccharose |
| LIU Li, ZHANG Yong-jun, XU Chang-zheng, LUO Feng |
| Research Center of Bioenergy & Bioremediation, Southwest University, Chongqing 400716, China |
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Abstract Due to fungal extracellular polysaccharide have high adsorption and high viscous characteristics, it is one of the difficulties of extracting the high purity of nucleic acids from extracellular polysaccharide germs. The production of scleroglucan strain Sclerotium rolfsii as the material, obtained high quality genomic DNA by the improved Cetyltrimethyl Ammonium Bromide (CTAB) method. By reducing the production of polysaccharide in the cell culture process to obtain high mycelium, relatively. And used the high concentration KAC and absolute ethyl alcohol to precipitate the crude polysaccharide, then the CTAB/NaCl solution to remove out the polysaccharide. Compared with the gDNA Kits and traditional method, highly yield and purity of gDNA obtained with simple steps. And the product is suitable for basic molecular experiments, such as building genome libraries and PCR.
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Received: 14 February 2014
Published: 25 May 2014
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