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| The Suspended Single Cell Culture and Embryogeny and Plant Regeneration of Pinellia ternata |
| OU Qiao-ming1, HOU Yi-qing1, BAO Mei-nian2, HU Da1, CHEN Yu-liang1, YUE Chun-ling 3 |
1. Bio-Technology Institute, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China;
2. School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730000, China;
3. The First Affiliated Hospital of Lanzhou University, Lanzhou 730000, China |
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Abstract After suspended cells as target material had been induced into polyploidy cell via COLC, it was a viable and effective polyploid induction approach that polyploidy cell was induced to differentiate and regenerate into homozygous polyploid. But the premise of the approach was that mature and efficient suspended cell culture and plant regeneration system has been set up. Base on good embryogenesis callus obtained, the key problems involved in suspended cells culture, cell differentiation and regeneration plant and its influence factors were studied. High quality suspended cell lines had been obtained which was primarily comprise of suborbicular cell. Cell growth curve showed a typical "S" curve, cell density was 2.0?106 above, living cells rate was 86.80%, suborbicular cell rate was 82.64%. The two possible approaches of the cell embryogeny of Pinellia ternata were clear and depinite. The embryogenic callus proliferated successfully by inducing cell differentiation and the efficient plant regeneration was realized finally. The differentiation of callus was 94.46%, rate of cluster buds induced was 337.42%, planting percent of buds was 90.75%, rooting rate of seedling was 95.40%, survival rate after transplanting was 98.83%. The system of high-efficiency suspended cell culture and plant regeneration were established. It provided technical reference and scientific basis to the follow-up study on polyploid induced of Pinellia ternata based on the single-cell level.
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Received: 16 May 2012
Published: 25 October 2012
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