人脂肪来源间充质干细胞植入受损耳蜗后定向归巢到并分化为毛细胞样细胞

doi:Q819

中国生物工程杂志

2010, Vol. 30

Issue (08): 1-5 DOI: Q819

研究报告

人脂肪来源间充质干细胞植入受损耳蜗后定向归巢到并分化为毛细胞样细胞

袁先道^1**,闫曦^2**,杨华¹,高志强^1***,李康华²,韩钦²,卢姗²,陈晓巍¹,亓放¹,姜鸿¹,赵春华^2***

1.中国医学科学院北京协和医学院北京协和医院耳鼻咽喉科北京 100730
2.中国医学科学院北京协和医学院基础医学院组织工程中心北京 100005

Human Adipose-derived Mesenchymal Stem Cells Engrafted into Deafened Mammalian Cochlea and Differentiated into Hair Cell-like Cells

YUAN Xian-dao¹,YAN Xi²,YANG Hua¹,GAO Zhi-qiang¹,LI Kang-hua²,HAN Qin²,LU Shan²,CHEN Xiao-wei¹,QI Fang¹,JIANG Hong¹,ZHAO Chun-hua²

1.Department of Otolaryngology, Peking Union Medical College Hospital， Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China
2.Institute of Basic Medical Sciences and School of Basic Medicine，Center of Excellence in Tissue Engineering，Chinese Academy of Medical Sciences and Peking Union Medical College，Beijing 100005，China

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摘要：

目的：通过将人脂肪来源间充质干细胞（human adipose-derived mesenchymal stem cells，hAD-MSCs）移植到受损小鼠，探讨hAD-MSCs替代缺失/受损毛细胞的可行性。方法：将hAD-MSCs经尾静脉移植入药物致聋后的小鼠体内，用免疫染色及RT-PCR等方法检测移植后hAD-MSCs在耳蜗内的归巢和分化。结果：移植的hAD-MSCs能够定向归巢到受损耳蜗内，并至少存活2周，未观察到对移植细胞的免疫排斥反应。有少量细胞定位于耳蜗感觉上皮并表达毛细胞特异性抗体myosin 7a。结论：hAD-MSCs移植入药物性致聋小鼠后，能够定向归巢到耳蜗内，并分化为内耳毛细胞样细胞，是一种针对内耳损伤及退变性疾病治疗的潜在细胞来源。

关键词： 耳蜗; 间充质干细胞; 耳聋; 异种移植

Abstract:

Objective: The aim was to examine the potential of human adipose-derived mesenchymal stem cells (hAD-MSCs) transplantation to restore inner ear hair cells in mice. Methods: To test this approach, hAD-MSCs were delivered into the cochlea of deafened mice. The grafted cells were tested by immunostaining and RT-PCR. Results: Transplanted cells survived for at least 2 weeks postoperatively, evidenced by RT-PCR and immunofluorescence detection of hAD-MSCs. A few hAD-MSCs located in the cochlear sensory epithelium, with expression of myosin 7a, a specific marker for inner ear hair cells. There was no evidence of significant immunological rejection of the implanted hAD-MSCs. Conclusion: hAD-MSCs transplanted into the mouse inner ear after drug-induced injury can survive, migrate and differentiate towards hair cell-like cells.

Key words: Cochlea Mesenchymal stem cells Hearing loss Xenotransplantation

收稿日期: 2009-12-21 出版日期: 2010-08-25

基金资助:

国家“十一五”“863”计划重大专项（2006AA02A109、2006AA02A115）、国家自然科学基金（30570771）、北京市科委重点项目（D07050701350701）、北京市科技新星计划（2006B53）资助项目

通讯作者: 高志强;赵春华 E-mail: zq.gao@yahoo.com;chunhuaz@public.tpt.tj.cn

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	袁先道
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	陈晓巍
	亓放
	姜鸿
	赵春华

引用本文:

袁先道闫曦杨华高志强李康华韩钦卢姗陈晓巍亓放姜鸿赵春华. 人脂肪来源间充质干细胞植入受损耳蜗后定向归巢到并分化为毛细胞样细胞[J]. 中国生物工程杂志, 2010, 30(08): 1-5.

YUAN Xian-Dao, YAN Xi, YANG Hua, GAO Zhi-Jiang, LI Kang-Hua, HAN Qin, LEI Pan, CHEN Xiao-Wei, QI Fang, JIANG Hong, DIAO Chun-Hua. Human Adipose-derived Mesenchymal Stem Cells Engrafted into Deafened Mammalian Cochlea and Differentiated into Hair Cell-like Cells. China Biotechnology, 2010, 30(08): 1-5.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/Q819 或 https://manu60.magtech.com.cn/biotech/CN/Y2010/V30/I08/1

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