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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
中国生物工程杂志  2021, Vol. 41 Issue (8): 103-109    DOI: 10.13523/j.cb.2104012
综述     
乳杆菌对致病假单胞菌的抑制作用研究进展*
孙瑶,乔梦伟,刘诗宇,宫殿良,宋金柱()
哈尔滨工业大学生命科学与技术学院 哈尔滨 150000
Research Progress on the Inhibitory Effect of Lactobacillus on Pathogenic Pseudomonas
SUN Yao,QIAO Meng-wei,LIU Shi-yu,GONG Dian-liang,SONG Jin-zhu()
School of Life Science and Technology, Harbin Institute of Technology, Harbin 150000,China
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摘要：

假单胞菌污染事件在临床就医和日常饮食中频发,屡次产生致病、致死等恶劣后果,有效抑制致病假单胞菌并降低其耐药性作为解决该问题的关键手段,是目前的研究重点。相关研究表明益生菌等天然活性成分对假单胞菌产生多方面影响,以应用范围最广的益生菌——乳杆菌为例,综合国内外最新研究进展,论述了乳杆菌对假单胞菌的生物膜结构、生长活性、生物毒性、黏附细胞表面能力及被假单胞菌感染后的小鼠等产生的影响。深入挖掘乳杆菌等益生菌及其代谢产物成分的作用机制,是防治假单胞菌等微生物污染和感染的关键。
关键词： 乳杆菌;  假单胞菌;  益生菌;  微生物防治    
Abstract:

Pseudomonas contamination incidents are not seldom encountered in clinical treatment and daily diet, which may result in illness, death and other undesirable consequences. To this end,recent studies focus on the inhibiting of pathogenic Pseudomonas and the reducing of their drug resistance, in which it has been proved that the excruciating characters of Pseudomonas can be improved by natural active ingredients (for example, probiotics). As a kind of widely existing probiotics, Lactobacillus is utilized for the survey. By considering the studies all over the world up to date, effects of Lactobacillus on the biofilm structure, growth activity, ability to adhere to cell surfaces and biological toxicity of Pseudomonas are introduced. Also, the influence of mice infected by Pseudomonas are is demonstrated. Based on the surveys aforementioned, it is significant to find out biological principles of influence on Pseudomonas of Lactobacillus and other probiotics (including their metabolites), which are the key to prevent and control microbial contamination and infection.
Key words: Lactobacillus    Pseudomonas    Probiotics    Microbiological control
收稿日期: 2021-04-09 出版日期: 2021-08-31
ZTFLH:  Q819  
基金资助: * 国家重点研发计划资助项目(2018YFD0200407)
通讯作者: 宋金柱     E-mail: sjz@hit.edu.cn
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孙瑶
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引用本文:

孙瑶,乔梦伟,刘诗宇,宫殿良,宋金柱. 乳杆菌对致病假单胞菌的抑制作用研究进展*[J]. 中国生物工程杂志, 2021, 41(8): 103-109.

SUN Yao,QIAO Meng-wei,LIU Shi-yu,GONG Dian-liang,SONG Jin-zhu. Research Progress on the Inhibitory Effect of Lactobacillus on Pathogenic Pseudomonas. China Biotechnology, 2021, 41(8): 103-109.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.2104012    或    https://manu60.magtech.com.cn/biotech/CN/Y2021/V41/I8/103

乳酸菌名称 细菌素 分离纯化方法 致病假单胞菌
名称		 抑制机制 文献
植物乳杆菌Lactobacillus plantarum B23 Lac-B23 SP Sepherose Fast Flow阳离子交换柱层析和Superdex Peptide 10/300 GL分子筛 荧光假单胞菌 破坏生物膜中的网络结构 [16]
面包乳杆菌Lactobacillus crustorum ZHG 2-1 ZHG 2-1粗提物CE 0.45 μm过滤器过滤、乙酸乙酯蒸馏、旋转蒸发、真空干燥制成粉末 铜绿假单胞菌 抑制生物膜的形成,降低预形成生物膜的厚度 [18]
罗伊氏乳杆菌Lactobacillus reuteri 罗伊氏素 离子交换树脂高效液相色谱(HPLC) 铜绿假单胞菌 抑制菌体生长,导致菌体死亡 [23]
植物乳杆菌Q7 Lactobacillus plantarum Q7 植物乳杆菌素Q7 吸附-解吸、CM-Fast Flow和Superdex Peptide 10/300 GL纯化 荧光假单胞菌 螯合锌离子后破坏菌体的外膜,引起钾离子和磷酸盐离子泄露 [24]
植物乳杆菌Lactobacillus plantarum B23 Lac-B23 SP Sepherose Fast Flow阳离子交换柱层析和Superdex Peptide 10/300 GL分子筛 荧光假单胞菌 假单胞菌株胞内物质的泄露,并破坏其细胞膜结构 [25]
植物乳杆菌Lactobacillus plantarum DL3 植物乳杆菌素DL3 乙酸乙酯提取蛋白质、Sephadex G50柱(1.6 cm×60 cm)色谱系统纯化 铜绿假单胞菌 破坏细胞壁,使得菌体中的蛋白质的泄漏,造成菌体死亡 [26]
植物乳杆菌Lactobacillus plantarum DY4-2 细菌素DY4-2 乙酸乙酯萃取、超滤、Sephadex G25色谱和高效液相色谱 荧光假单胞菌、
铜绿假单胞菌		 降低总活菌数和总挥发性盐基氮(TVB-N) [27]
副干酪乳杆菌Lactobacillus paracasei FX-6 抗菌物质 上清液中分子量低于6 000的组分、超滤法进行冷冻干燥 恶臭假单胞菌 提高细菌细胞膜的通透性,破坏菌的细胞形态,影响其蛋白质的合成 [28]、
[29]
表1  乳杆菌细菌素对致病假单胞菌的抑制
细菌名称 抑制活性的百分比/%
48 h 72 h
植物乳杆菌(Lactobacillus plantarium) 40 80
嗜酸乳杆菌(Lactobacillus acidophilus) 40 90
干酪乳杆菌(Lactobacillus casei) 10 90
发酵乳杆菌(Lactobacillus fermentum) 60 20
罗伊氏乳杆菌(Lactobacillus reuteri) 20 70
加氏乳杆菌(Lactobacillus gasseri) 30 70
嗜酸乳杆菌(Lactobacillus acidophilus) (商业) 0 0
H2O2 3% 0 100
乳酸 3% 0 100
表2  部分乳杆菌培养上清48 h和72 h对多重耐药铜绿假单胞菌的抑制活性[32]
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