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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
中国生物工程杂志  2018, Vol. 38 Issue (2): 82-88    DOI: 10.13523/j.cb.20180212
综述     
环状RNA的结构和功能特性及其与胃癌发生的关系 *
姚立鹏1,2,葛炜1,胡英君1,骆海燕1,吴珊珊1,林飞蕾1,郭俊明2*()
1 宁波卫生职业技术学院 宁波 315000
2 宁波大学医学院生物化学与分子生物学研究所 浙江省病理生理学技术研究重点实验室 宁波 315211
The Structural and Functional Characteristics of Circular RNAs and Their Relationships with Gastric Cancer
Li-peng YAO1,2,Wei GE1,Ying-jun HU1,Hai-yan LUO1,Shan-shan WU1,Fei-lei LIN1,Jun-ming GUO2*()
1 Ningbo College of Health Sciences,Ningbo 315000, China
2 Department of Biochemistry and Molecular Biology, Zhejiang Key Laboratory of Pathophysiology,Medical School of Ningbo University, Ningbo 315211, China
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摘要：

环状 RNA是一类新发现以共价键形成环状结构的RNA分子,没有5'帽和3'尾,具有高度的序列保守性和稳定性,不易被核酸外切酶降解,在转录或转录后水平发挥基因表达调控的作用。近年来,已发现环状RNA与许多肿瘤包括胃癌发生密切相关。首先介绍环状 RNA形成、分类、分子特征和功能,然后从环状 RNA与胃癌发生、环状 RNA与胃癌诊断、以及环状 RNA在胃癌治疗中的作用等三个方面阐述环状RNA与胃癌的关系。
关键词： 环状;  RNA;  胃癌;  基因表达;  肿瘤    
Abstract:

Circular RNAs (circRNAs) are a newly discovered RNA molecules, which are widely distributed in mammalian cells. They do not have 5'cap and 3' tail, but have a high degree of sequence conservation and stability, and not easy to be degraded by RNA exonuclease. What’s more, they not only can regulate the expression of genes, but also play a regulatory role at transcriptional or transcriptional levels. In recent years, circRNAs have been found to be associated with many tumors including gastric cancer. The formation, classification, molecular characterization and function of circRNAs were introduced. Then, the relationships between circRNAs and gastric cancer are analyzed from three aspects: the correlations between circRNAs and gastric cancer occurrence, the values of circRNAs in the diagnosis of gastric cancer, and the roles of circRNAs in the treatment of gastric cancer.
Key words: circRNA    Gastric cancer    Gene expression    Tumor
收稿日期: 2017-10-30 出版日期: 2018-03-21
ZTFLH:  Q819  
基金资助: 国家自然科学基金(81772279);浙江省公益技术研究社会发展(2016C33177);宁波市科技创新团队项目(2017C110019);浙江省病理生理学技术研究重点实验室开放基金资助项目(201701)
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姚立鹏
葛炜
胡英君
骆海燕
吴珊珊
林飞蕾
郭俊明

引用本文:

姚立鹏,葛炜,胡英君,骆海燕,吴珊珊,林飞蕾,郭俊明. 环状RNA的结构和功能特性及其与胃癌发生的关系 *[J]. 中国生物工程杂志, 2018, 38(2): 82-88.

Li-peng YAO,Wei GE,Ying-jun HU,Hai-yan LUO,Shan-shan WU,Fei-lei LIN,Jun-ming GUO. The Structural and Functional Characteristics of Circular RNAs and Their Relationships with Gastric Cancer. China Biotechnology, 2018, 38(2): 82-88.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20180212    或    https://manu60.magtech.com.cn/biotech/CN/Y2018/V38/I2/82

图1  环状 RNA 的生成方式
Species Sequencing samples CircRNAs’ number References
Homo sapiens fetal head 1184 [9]
19 and 37 weeks female fetal cerebellum 21071
20 and 22 weeks male fetuses diencephalon 24632
20 and 22 weeks female fetal frontal lobe 38983
22 weeks male fetuses and
20 weeks female fetuses occipital lobe 31085
24 weeks female fetuses and
22 weeks parietal male fetuses 23303
20 and 24 weeks female fetuses temporal lobe 21835 [10]
Human neuroblastoma cells SH-SY5Y 4264 [15]
Human fibroblasts Hs68 cell line 7771
H9 cell line 103
Cell line including A549,AG04450,BJ,GM12878,H1-hESC,HeLa S3,HepG2, HMEC, HSMM, HUVEC, K562, MCF-7, NHEK, NHLF and SK-N-SHRA 181535
CD19+, CD34+, HEK293 and Neutrophil cell line 2487 [4]
cell-free saliva, CFS >400 [16]
表1  人类细胞中已鉴定出的circRNA数量
图2  环状 RNA的主要功能
circRNA Sample
type		 Sample number Expression
in tumors		 Clinical
correlation		 Function Methods Reference
hsa_circ_
002059		 Tissue/
Plasma		 101 Paired cancer tissues and adjacent nontumorous tissues; 36 paired plasma samples from preoperative and postoperative patients Down Distal metastasis, TNM stage, Gender and age qRT-PCR [37]
hsa_circ_
0001649		 Tissue/
plasma		 76 Tumor tissue samples and their paired paracancerous histological normal tissues; 20 patients’ whole blood samples preoperatively and postoperatively Down Pathological differentiation qRT-PCR [32]
hsa_circ_
0000190		 Tissue/
plasma		 104 paired cancer tissues and adjacent non-tumor tissues; 104 plasma samples from cancer patients or health controls Down Tumor diameter, lymphatic metastasis,distal metastasis, TNM stage and CA19-9 levels qRT-PCR [33]
hsa_circ_
0000096		 Tissue 101 paired cancer tissues and adjacent non-tumorous tissues Down Promotes cancer cell growth and migration by regulating cyclin D1, CDK6, MMP-2 and MMP-9 qRT-PCR [34]
circPVT1 Tissue 187 Paired cancer specimens and adjacent normal tissues Up An independent prognostic marker for overall survival and disease-free survival Promote cell proliferation by acting as a sponge for miR-125 family members qRT-PCR [43]
hsa_circ_
0003159		 Tissue 108 paired gastric cancer tissues and adjacent non- tumorous tissues Down Gender, distal metastasis,tumor-node-metastasis stage qRT-PCR [46]
hsa_circ_
0001895		 Tissue 96 gastric cancer tissues and their adjacent non-tumorous tissues,35 healthy gastric mucosa and 30 gastric dysplasia tissues Down Cell differentiation, Borrmann type, tissue carcinoembryonic antigen expression qRT-PCR [47]
hsa_circ_
0006633		 Tissue/
plasma		 96 gastric cancer tissues and their adjacent non-tumorous tissues,35 human healthy gastric mucosa, 51 gastritis mucosa, and 20 gastric dysplasia tissues;Plasma samples were obtained from 20 healthy volunteers and 20 gastric cancer patients Down Distal metastasis,tissue CEA level qRT-PCR [48]
  
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